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Simultaneous Determination of 19 Antibiotics and 19 Anthelmintics Residues in Edible Fungi by UHPLC-MS/MS in Combination with QuEChERS Method

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Abstract

A novel sensitive method for simultaneous determination of 19 antibiotics and 19 anthelmintics in edible fungi using QuEChERS-UHPLC-MS/MS method was established. For every 10.0 g sample, 20 mL of acetonitrile with 2% acetic acid and 1.2 g sodium acetate were used for extraction, followed by 6.0 g anhydrous MgSO4 for dehydration, and 50 mg anhydrous MgSO4+10 mg PSA for purification. The optimized QuEChERS method presented excellent recoveries ranging from 85.31% to 103.94%, with relative standard deviations of 3.65–18.31%. Limit of detection and limit of quantification of the analysis method were 0.24–19 ng/L and 0.8–65 ng/L, respectively. The method was applied to analyze the 38 target compounds in 8 kinds of edible fungi samples from markets in Chengdu, China. The results indicated that the antibiotics and anthelmintics were widely existed with concentrations ranging from below LOD to 296 μg·L−1. The concentrations of sulfamethoxazole, sulfadiazine, thiabendazole and fenbendazole were relatively high. Based on the current study, exposure to antibiotics and anthelmintics through edible fungi consumption is harmless to human. However, long term exposure to low doses of antibiotics and anthelmintics still needs attention and merits further study.

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Funding

This study was supported by the College Students’ Innovative Entrepreneurial Training Plan Program of China (No. C2019104662).

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Authors and Affiliations

  1. College of Architecture and Environment, Sichuan University, Chengdu, 610065, China

    Shu Zhu, Meng Gao, Sisi Tian, Yaqin Bu, Zhiwei Gan, Xuan Ma, Yiwen Li & Zhi Li

  2. College of Life Sciences, Sichuan University, Chengdu, 610065, China

    Hengjie Cui

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  1. Shu Zhu
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  2. Meng Gao
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  3. Sisi Tian
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  4. Yaqin Bu
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  7. Xuan Ma
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  8. Yiwen Li
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  9. Zhi Li
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Correspondence to Zhi Li.

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Zhu, S., Gao, M., Tian, S. et al. Simultaneous Determination of 19 Antibiotics and 19 Anthelmintics Residues in Edible Fungi by UHPLC-MS/MS in Combination with QuEChERS Method. Food Anal. Methods 14, 1278–1288 (2021). https://doi.org/10.1007/s12161-020-01949-3

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