Abstract
A matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) method were established to detect Campylobacter jejuni and Listeria monocytogenes from a chicken farm and samples from commercial live chickens. Suspected isolates were pretreated and crystallized by matrix solutions consisting of ethanol, acetonitrile, and water with different proportions. The effect of matrix composition was evaluated by examining the differences in characteristic peak values and relative abundances. Significantly different matrix solutions were needed for the detection of C. jejuni and L. monocytogenes: the former did not require lysis by formic acid and was sensitive to trifluoroacetic acid (TFA), but the latter was just the opposite. Control of the water content in matrix solution, TFA concentration, and matrix concentration will enhance the number and relative abundance of characteristic peaks and improve the detection rate. An optimized pretreatment enables the rapid identification of foodborne pathogens, which will make it possible to improve the timeliness of disease prevention and foodborne risk monitoring.
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Acknowledgments
The authors thank the technical supports by the BioMerieux and the cooperative demonstration laboratories of BioMerieux. The linguistic editing and proofreading provided by TopEdit LLC during the preparation of this manuscript are acknowledged.
Funding
This study was funded by the 16th group of Six Talent Climax Foundation of Jiangsu Project (SWYY-230), general scientific research programs of Jiangsu Committee on Health and Hygiene (H2018098), technology support program (social development) of Changzhou (CE20185048), and National Natural Science Foundation of China (Grant No. 21607016).
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Tu Bowen declares no conflict of interest. Xue Yingang declares no conflict of interest. Jingchang Xue declares no conflict of interest. Du Qiang declares no conflict of interest. Li Junhong declares no conflict of interest. Tang Hongbing declares no conflict of interest. Han Xiaodong declares no conflict of interest.
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Bowen, T., Yingang, X., Xue, J. et al. Rapid Detection of Microbial Mass Spectra VITEK-MS for Campylobacter jejuni and Listeria monocytogenes. Food Anal. Methods 13, 412–419 (2020). https://doi.org/10.1007/s12161-019-01663-9
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DOI: https://doi.org/10.1007/s12161-019-01663-9