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Method Validation for the Determination of Mercury, Cadmium, Lead, Arsenic, Copper, Iron, and Zinc in Fish Through Microwave-Induced Plasma Optical Emission Spectrometry (MIP OES)

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Abstract

A simple, sensitive, and selective analytical method was validated for the determination of mercury, cadmium, lead, arsenic, copper, iron, and zinc in fish muscle using microwave-induced plasma optical emission spectrometry (MIP OES) after acid digestion. The procedure for determining linear range, detection and quantification limits, selectivity, trueness, repeatability, reproducibility, and uncertainty of the method is reported. The results of the validation process demonstrate that the method complies with the provisions of the European Commission guidelines for metals mercury, arsenic, copper, iron, and zinc, but for cadmium and lead, it can only be used as a screening method. The SRM 1946 (Lake Superior Fish Tissue) certified reference material was used to evaluate analytical result quality. The recovery varies between 87 and 99.7%. The HorRat value was equal to or lower than 0.67, and the expanded uncertainty was lower than 37%. The method described can be considered adequate for the simultaneous determination and quantification of the chosen heavy metals in fish matrices. For Cd and Pb, the method could only be employed as a screening tool and not for official control of these contaminants.

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Acknowledgements

This article was supported by the Diagnosis and Pollution Control Research Group (GDCON), Faculty of Engineering, University Research Center (SIU).

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Authors and Affiliations

  1. Impact of Food Components on Health (ICAS), Escuela de Nutrición y Dietética, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Colombia

    Sara Elisa Gallego Ríos & Claudia María Ramírez Botero

  2. Diagnosis and Pollution Control Research Group (GDCON), Faculty of Engineering, University Research Center (SIU), Universidad de Antioquia, Calle 70 No. 52-21, Medellin, Colombia

    Gustavo A. Peñuela

Authors
  1. Sara Elisa Gallego Ríos
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  2. Gustavo A. Peñuela
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  3. Claudia María Ramírez Botero
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Correspondence to Sara Elisa Gallego Ríos.

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Funding

This work was supported by the 2015–2016 sustainability fund.

Conflict of Interest

Sara Elisa Gallego Ríos declares that she has no conflict of interest. Gustavo A. Peñuela declares that he has no conflict of interest. Claudia María Ramírez Botero declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Gallego Ríos, S.E., Peñuela, G.A. & Ramírez Botero, C.M. Method Validation for the Determination of Mercury, Cadmium, Lead, Arsenic, Copper, Iron, and Zinc in Fish Through Microwave-Induced Plasma Optical Emission Spectrometry (MIP OES). Food Anal. Methods 10, 3407–3414 (2017). https://doi.org/10.1007/s12161-017-0908-0

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