Abstract
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) expresses at high level in many cancers and has been suggested as a potential therapeutic target. It was reported that the Kringle (KNG) domain of ROR1 extracellular region is involved in ROR1/ROR2 heterooligomerization. Monoantibodies that target KNG domain of ROR1 could induce apoptosis of chronic lymphocytic leukemia cells. Here we present the backbone and side chain assignments of KNG domain of ROR1, which lays a foundation for its further structural and function research.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Change history
28 June 2018
The authors would like to include an additional institution to their affiliation which was initially not included in the original publication of the article
References
Battistel MD, Grishaev A, An SS, Castellino FJ, Llinas M (2009) Solution structure and functional characterization of human plasminogen kringle 5. Biochemistry 48:10208–10219. https://doi.org/10.1021/bi901433n
Daneshmanesh AH et al (2012) Monoclonal antibodies against ROR1 induce apoptosis of chronic lymphocytic leukemia (CLL) cells. Leukemia 26:1348–1355. https://doi.org/10.1038/leu.2011.362
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293
Fernandez NB et al (2016) ROR1 contributes to melanoma cell growth and migration by regulating N-cadherin expression via the PI3K/Akt pathway. Mol Carcinog 55:1772–1785. https://doi.org/10.1002/mc.22426
Forrester WC (2002) The Ror receptor tyrosine kinase family. Cell Mol Life Sci 59:83–96
Fukuda T et al (2008) Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a. Proc Natl Acad Sci USA 105:3047–3052. https://doi.org/10.1073/pnas.0712148105
Hasan MK et al (2017) Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells. Leukemia. https://doi.org/10.1038/leu.2017.133
Hojjat-Farsangi M et al (2013) The tyrosine kinase receptor ROR1 is constitutively phosphorylated in chronic lymphocytic leukemia (CLL) Cells. PLoS ONE. https://doi.org/10.1371/journal.pone.0078339
Hojjat-Farsangi M, Moshfegh A, Daneshmanesh AH, Khan AS, Mikaelsson E, Osterborg A, Mellstedt H (2014) The receptor tyrosine kinase ROR1-An oncofetal antigen for targeted cancer therapy. Semin Cancer Biol 29:21–31. https://doi.org/10.1016/j.semcancer.2014.07.005
Ida L, Yamaguchi T, Yanagisawa K, Kajino T, Shimada Y, Suzuki M, Takahashi T (2016) Receptor tyrosine kinase-like orphan receptor 1, a target of NKX2–1/TTF-1 lineage-survival oncogene, inhibits apoptosis signal-regulating kinase 1-mediated pro-apoptotic signaling in lung adenocarcinoma. Cancer Sci 107:155–161. https://doi.org/10.1111/cas.12858
Kovacs H, Gossert A (2014) Improved NMR experiments with 13C-isotropic mixing for assignment of aromatic and aliphatic side chains in labeled proteins. J Biomol NMR 58:101–112. https://doi.org/10.1007/s10858-013-9808-9
Liu Y, Yang H, Chen T, Luo Y, Xu Z, Li Y, Yang J (2015) Silencing of receptor tyrosine kinase ROR1 inhibits tumor-cell proliferation via PI3K/AKT/mTOR signaling pathway in lung adenocarcinoma. PLoS ONE 10:e0127092. https://doi.org/10.1371/journal.pone.0127092
Maderegger B, Bermel W, Hrzenjak A, Kostner GM, Sterk H (2002) Solution structure of human apolipoprotein(a) kringle IV type 6. Biochemistry 41:660–668
Masiakowski P, Carroll RD (1992) A novel family of cell surface receptors with tyrosine kinase-like domain. J Biol Chem 267:26181–26190
Minami Y, Oishi I, Endo M, Nishita M (2010) Ror-family receptor tyrosine kinases in noncanonical Wnt signaling: their implications in developmental morphogenesis and human diseases. Dev Dyn 239:1–15. https://doi.org/10.1002/dvdy.21991
Potratz J et al (2016) Receptor tyrosine kinase gene expression profiles of Ewing sarcomas reveal ROR1 as a potential therapeutic target in metastatic disease. Mol Oncol 10:677–692. https://doi.org/10.1016/j.molonc.2015.12.009
Uhrmacher S, Schmidt C, Erdfelder F, Poll-Wolbeck SJ, Gehrke I, Hallek M, Kreuzer KA (2011) Use of the receptor tyrosine kinase-like orphan receptor 1 (ROR1) as a diagnostic tool in chronic lymphocytic leukemia (CLL). Leuk Res 35:1360–1366. https://doi.org/10.1016/j.leukres.2011.04.006
Yu J et al (2016) Wnt5a induces ROR1/ROR2 heterooligomerization to enhance leukemia chemotaxis and proliferation. J Clin Investig 126:585–598. https://doi.org/10.1172/JCI83535
Zhang S et al (2012a) The onco-embryonic antigen ROR1 is expressed by a variety of human cancers. Am J Pathol 181:1903–1910. https://doi.org/10.1016/j.ajpath.2012.08.024
Zhang SP et al (2012b) ROR1 Is expressed in human breast cancer and associated with enhanced tumor-cell growth. PLoS ONE. https://doi.org/10.1371/journal.pone.0031127
Zhang HL et al (2014a) ROR1 expression correlated with poor clinical outcome in human ovarian cancer. Sci Rep. https://doi.org/10.1038/srep05811
Zhang SP et al (2014b) Ovarian cancer stem cells express ROR1, which can be targeted for anti-cancer-stem-cell therapy. Proc Natl Acad Sci USA 111:17266–17271. https://doi.org/10.1073/pnas.1419599111
Acknowledgements
This work was supported by the National Key Research and Development Program of China (No. 2017YFC0906903), the National Natural Science Foundation of China (No. 81401904, 31400642) and Yunnan Provincial Science and Technology Department (No. 2015FB170).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare no conflict of interest.
Rights and permissions
About this article
Cite this article
Ma, X., Zhang, Y., Liu, B. et al. Backbone and side-chain chemical shift assignments of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (ROR1). Biomol NMR Assign 12, 145–148 (2018). https://doi.org/10.1007/s12104-017-9797-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-017-9797-9