Abstract
Cyclophilins are peptidyl prolyl isomerases that play an important role in a wide variety of biological functions like protein folding and trafficking, intracellular and extracellular signaling pathways, nuclear translocation and in pre-mRNA splicing. Two cyclophilins have been identified in the parasitic organism Trichomonas vaginalis and were named as TvCyP1 and TvCyP2. The 2 enzymes have been found to interact with Myb transcription factors in the parasite which regulate the iron induced expression of ap65-1 gene leading to cytoadherence of the parasite to human vaginal epithelial cells to cause the disease trichomoniasis. TvCyP2 was found to interact specifically with Myb3 to regulate nuclear translocation of the transcription factor. It would be intriguing to identify the binding site of both proteins as it could pave way to newer targets for drug discovery. Here we report the 1H, 13C and 15N resonance assignments and secondary structure information of TvCyP2 that could help us investigate the interaction between Myb3 and TvCyP2 in detail using NMR.
Similar content being viewed by others
References
Dornan J, Page AP, Taylor P, Wu S, Winter AD, Husi H, Walkinshaw MD (1999) Biochemical and structural characterization of a divergent loop cyclophilin from Caenorhabditis elegans. J Biol Chem 274:34877–34883
Dunne RL, Dunn LA, Upcroft P, O’Donoghue PJ, Upcroft JA (2003) Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis. Cell Res 13:239–249
Fichorova RN (2009) Impact of T. vaginalis infection on innate immune responses and reproductive outcome. J Reprod Immunol 83:185–189
Hsu HM, Ong SJ, Lee MC, Tai JH (2009) Transcriptional regulation of an iron-inducible gene by differential and alternate promoter entries of multiple Myb proteins in the protozoan parasite Trichomonas vaginalis. Eukaryot Cell 8(3):362–372
Hsu HM, Chu CH, Wang YT, Lee Y, Wei SY, Liu HW, Ong SJ, Chen C, Tai JH (2014) Regulation of nuclear translocation of the Myb1 transcription factor by TvCyclophilin 1 in the protozoan parasite Trichomonas vaginalis. J Biol Chem 289:19120–19136
Kay LE (1995) Pulsed field gradient multi-dimensional NMR methods for the study of protein structure and dynamics in solution. Prog Biophys Mol Bio 63:277–299
Menezes CB, Frasson AP, Tasca T (2016). Trichomoniasis—are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide? Microb Cell 3:404–418
Nigro P, Pompilio G, Capogrossi MC (2013) Cyclophilin A: a key player for human disease. Cell Death Dis 4(10):e888
Pervushin K, Riek R, Wider G, Wuthrich K (1998) Transverse relaxation-optimized spectroscopy (TROSY) for NMR studies of aromatic spin systems in C-13-labeled proteins. J Am Chem Soc 120:6394–6400
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol Nmr 44(4):213–223
Wang P, Heitman J (2005) The cyclophilins. Genome Biol 6:226
Acknowledgements
The NMR spectra were obtained at the High-field Biomacromolecular NMR Core Facility, Academia Sinica, supported by Core Facilities for Protein Structural Analysis from National Research Program for Biopharmaceuticals (NRPB). This work is supported by Academia Sinica and the Ministry of Science and Technology, Taiwan, ROC (MOST 103-2311-B-001-026-MY3 and MOST 105-2320-B-001-019-MY3).
Author information
Authors and Affiliations
Corresponding author
Additional information
Tesmine Martin and Yuan-Chao Lou have contributed equally to this work.
Rights and permissions
About this article
Cite this article
Martin, T., Lou, YC., Aryal, S. et al. 1H, 13C and 15N resonance assignments and secondary structures of cyclophilin 2 from Trichomonas vaginalis . Biomol NMR Assign 12, 27–30 (2018). https://doi.org/10.1007/s12104-017-9774-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12104-017-9774-3