Abstract
The SPOUT family of methyltransferase proteins is noted for containing a deep trefoil knot in their defining backbone fold. This unique fold is of high interest for furthering the understanding of knots in proteins. Here, we report the 1H, 13C, 15N assignments for MTT Tm , a canonical member of the SPOUT family. This protein is unique, as it is one of the smallest members of the family, making it an ideal system for probing the unique properties of the knot. Our present work represents the foundation for further studies into the topology of MTT Tm , and understanding how its structure affects both its folding and function.
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Acknowledgements
We would like to thank Dominique Capraro and Xuemei Huang for insightful scientific discussions and expertise on NMR. This work was supported by the UCSD Molecular Biophysics Training Program (National Institutes of Health GM008326) and the San Diego Fellowship (D. J. B.) and funded by the National Science Foundation (PHY-1614407 and PHY-1212312 to P.A.J.).
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Burban, D.J., Jennings, P.A. Backbone assignments for the SPOUT methyltransferase MTT Tm , a knotted protein from Thermotoga maritima . Biomol NMR Assign 11, 151–154 (2017). https://doi.org/10.1007/s12104-017-9737-8
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DOI: https://doi.org/10.1007/s12104-017-9737-8