Abstract
The regulation and localization of the neuronal voltage gated Ca2+ channel CaV1.2 is important for synaptic plasticity associated with learning and memory. The cytoskeletal protein, α-actinin-1 is known to interact with CaV1.2 and stabilize its localization at the postsynaptic membrane. Here we report both backbone and sidechain NMR assignments for the C-terminal EF-hands (EF3 and EF4) of α-actinin-1 (residues 824–892, called ACTN_EF34) bound to the IQ-motif (residues 1644–1665) from CaV1.2 (BMRB accession no. 25902).
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References
Atkinson RA, Joseph C, Kelly G, Muskett FW, Frenkiel TA, Nietlispach D, Pastore A (2001) Ca2+-independent binding of an EF-hand domain to a novel motif in the alpha-actinin–titin complex. Nat Struct Biol 8:853–857
Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeiffer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277–293
Gao T, Bunemann M, Gerhardstein BL, Ma H, Hosey MM (2000) Role of the C terminus of the alpha 1C (CaV1.2) subunit in membrane targeting of cardiac L-type calcium channels. J Biol Chem 275:25436–25444
Hall DD, Dai S, Tseng PY, Malik Z, Nguyen M, Matt L, Schnizler K, Shephard A, Mohapatra DP, Tsuruta F, Dolmetsch RE, Christel CJ, Lee A, Burette A, Weinberg RJ, Hell JW (2013) Competition between a-actinin and Ca2+-calmodulin controls surface retention of the L-type Ca2+ channel Ca(V)1.2. Neuron 78:483–497
Hell JW, Westenbroek RE, Warner C, Ahlijanian MK, Prystay W, Gilbert MM, Snutch TP, Catterall WA (1993) Identification and differential subcellular localization of the neuronal class C and class D L-type calcium channel alpha 1 subunits. J Cell Biol 123:949–962
Ikura M, Kay LE, Bax A (1990) A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin. Biochemistry 29:4659–4667
Lee W, Tonelli M, Markley JL (2015) NMRFAM-SPARKY: enhanced software for biomolecular NMR spectroscopy. Bioinformatics 31:1325–1327
Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44:213–223
Turner M, Zhang Y, Carlson HL, Stadler HS, Ames JB (2015) Chemical shift assignments of mouse HOXD13 DNA binding domain bound to duplex DNA. Biomol NMR Assign 9:267–270
Vogl AM, Brockmann MM, Giusti SA, Maccarrone G, Vercelli CA, Bauder CA, Richter JS, Roselli F, Hafner AS, Dedic N, Wotjak CT, Vogt-Weisenhorn DM, Choquet D, Turck CW, Stein V, Deussing JM, Refojo D (2015) Neddylation inhibition impairs spine development, destabilizes synapses and deteriorates cognition. Nat Neurosci 18:239–251
Wishart DS, Sykes BD, Richards FM (1992) The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry 31:1647–1651
Acknowledgments
We thank Jeff Walton and Bennett Addison for technical support and help with NMR experiments. Work supported by NIH Grants (EY012347) to J.B.A. and (AG017502) to J.W.H.
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Turner, M., Anderson, D.E., Rajan, S. et al. Chemical shift assignments of the C-terminal EF-hand domain of α-actinin-1. Biomol NMR Assign 10, 219–222 (2016). https://doi.org/10.1007/s12104-016-9670-2
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DOI: https://doi.org/10.1007/s12104-016-9670-2