Abstract
Restorer line F6 (Oryza sativa L. ssp. indica) has been widely used in hybrid rice breeding systems in southern China. However, line F6 is susceptible to drought stress, which restricts its utilization in many areas. Dongxiang wild rice (DXWR, Oryza rufipogon Griff.) has strong drought stress resistance, but the molecular factors responsible for drought resistance in DXWR remain unknown. In this study, we used the combination of phenotypic selection and molecular marker-assisted selection (MAS) to improve the drought stress resistance of line F6 by introgression of qSDT12-2, a large effect drought stress-related quantitative trait locus identified in DXWR. Molecular MAS was carried out using linked marker RM1226, which is associated with qSDT12-2. Genomic background assessment was performed using 112 polymorphic markers. Finally, a stable drought stress-resistant backcross inbred line (BIL) was developed from a \(\hbox {BC}_{{5}} \hbox {F}_{{5}}\) population, termed BIL627. Genetic constitution analysis revealed that the genome of BIL627 is almost identical (99.1%) to that of the restorer line F6. Further, BIL627 showed no yield penalty and no decrease in restoration ability under normal conditions. Taken together, our findings reveal the intrinsic value of using genetic resources present in wild species of Oryza to improve undesirable traits found in cultivated rice.
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Acknowledgements
We would like to thank all the members of the project who contributed to the field work. This research was supported by the National Natural Science Foundation of China (31660386), the Natural Science Foundation of Jiangxi Province for Distinguished Young Scholars (20171BCB23040), the Natural Science Foundation of Jiangxi Province, China (20181BAB204010), the Foundation of Jiangxi Educational Committee (GJJ170193) and the Sponsored Programme for Distinguished Young Scholars at Jiangxi Normal University.
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Luo, Y., Lao, L., Ai, B. et al. Development of a drought stress-resistant rice restorer line through Oryza sativa–rufipogon hybridization. J Genet 98, 55 (2019). https://doi.org/10.1007/s12041-019-1105-2
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DOI: https://doi.org/10.1007/s12041-019-1105-2