Skip to main content
SpringerLink
Log in

A microscopic study on scattering in tissue section of Alternanthera philoxeroides under polarized light

  • Published:
Journal of Biosciences Aims and scope Submit manuscript

Abstract

Like any other biological tissue, plant tissue also exhibits optical properties like refraction, transmission, absorption, coloration, scattering and so on. Several studies have been conducted using different parts of plants such as leaves, seedlings, roots, stems and so on, and their optical properties have been analyzed to study plant physiology, influence of environmental cues on plant metabolism, light propagation through plant parts and the like. Thus, it is essential to study in detail the optical properties of several plant parts to determine their structural relationship. In this backdrop, an experimental study was conducted to observe and analyze the optical properties of node and inter-nodal tissue cross-sections of the plant Alternanthera philoxeroides under a polarizing microscope constructed and standardized in the laboratory. The observed optical properties of the microscopic tissue sections have been then studied to determine a significant structural relationship between nodal and inter-nodal tissue arrangement patterns as a whole. Tissue sections that have undergone a sort of biological perturbation like loss of water (dried in air for 15 min) have also been studied to study the change in the pattern of tissue optical property when compared with that of normal plant-tissue cross-sections under a polarizing microscope. This type of biological perturbation was chosen for the study because water plays an important role in maintenance of the normal physiological processes in plants and most other forms of life.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2

Similar content being viewed by others

References

  • Ansari MA and Mohajerani E 2011 Mechanisms of laser-tissue interaction: optical properties of tissue. Lasers Med. Sci. 2 119–125

    Google Scholar 

  • Chance B, Cope M, Gratton E, Ramanujam N and Tromberg B 1998 Phase measurement of light absorption and scatter in human tissue. Rev. Sci. Instrum. 693 457–3481

    Google Scholar 

  • Datt B 1999 A new reflectance index for remote sensing of chlorophyll content in higher plants: tests using Eucalyptus leaves. J. Plant Physiol. 154 30–36

    Article  CAS  Google Scholar 

  • Esau K 1977 Anatomy of Seed Plants (New York: J Wiley & Sons)

    Google Scholar 

  • Fahn A 1990 Plant Anatomy (United Kingdom: Oxford Press)

    Google Scholar 

  • Hochberg U, Windt CW, Ponomarenko A, Zhang YJ, Gersony J, Rockwell FE and Holbrook NM 2017 Stomatal closure, basal leaf embolism, and shedding protect the hydraulic integrity of grape stems. Plant Physiol. 174 764–775

    Article  CAS  Google Scholar 

  • Jacquemoud S, Ustin SL, Verdebout J, Schmuck G, Andreoli G and Hosgood B 1996 Estimating leaf biochemistry using the PROSPECT leaf optical properties model.Remote Sens. Environ. 56 194–202

    Google Scholar 

  • Jacques SL 2013 Optical properties of biological tissues: a review. Phys. Med. Biol. 58 37–61

    Article  Google Scholar 

  • Kerker M 2013 Introduction; in The scattering of Light and other Electromagnetic Radiation: Physical Chemistry: A Series of Monographs (ed) Loebl ME (United States of America: Academic Press)

    Google Scholar 

  • Lee DW 2009 Plant tissue optics: micro-and nanostructures. In Biomimetics and Bioinspiration 7401 740104. International Society for Optics and Photonics

  • Mandoli DF and Briggs WR 1982 Optical properties of etiolated plant tissues. Proc. Natl. Acad. Sci. 79 2902–2906

  • Oldenbourg R 2013 Polarized light microscopy: principles and practice. Cold Spring Harb. Protoc. 11 1023–1036

    Google Scholar 

  • Preuss LE and Profio AE 1989 Optical properties of mammalian tissue: introduction by the feature editors. Appl. Opt. 28 2207–2209

    Article  CAS  Google Scholar 

  • Rühr PT and Lambertz M 2019 Surface contrast enhancement of integumentary structures in X-ray tomography. J. Anat. 235 379–385

    Article  Google Scholar 

  • Sun Q, Yoda K and Suzuki H 2004 Internal axial light conduction in the stems and roots of herbaceous plants. J. Exp. Bot. 56 191–203

    PubMed  Google Scholar 

  • Tuchin VV 2007 Tissue Optics: Light Scattering Methods and Instruments for Medical Diagnosis (Washington: Society of Photo-Optical Instrumentation Engineers)

    Book  Google Scholar 

  • Tucker CJ and Sellers PJ 1986 Satellite remote sensing of primary production. Int. J. Remote Sens. 7 1395–1416

    Article  Google Scholar 

  • Vogelmann TC 1993 Plant tissue optics. Annu. Rev. Plant Biol. 44 231–251

    Article  Google Scholar 

Download references

Acknowledgements

This project is funded by Cognitive Science Research Initiative (CSRI) of Department of Science and Technology, India. The authors remain grateful to faculty members of the Biological Sciences Division of Indian Statistical Institute, Kolkata.

Author information

Authors and Affiliations

  1. Laboratory for Cognitive Systems and Cybernetics Research, Center for Soft Computing Research, Indian Statistical Institute, Kolkata, 700 108, India

    Shibsankar Roy, Barnini Bhattacharya, Bijay Bal & Kuntal Ghosh

  2. Department of Physiology, University of Calcutta, Kolkata, India

    Barnini Bhattacharya

  3. Saha Institute of Nuclear Physics (Retired), Kolkata, India

    Bijay Bal

  4. Machine Intelligence Unit, Indian Statistical Institute, Kolkata, India

    Kuntal Ghosh

Authors
  1. Shibsankar Roy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Barnini Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bijay Bal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kuntal Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kuntal Ghosh.

Additional information

Communicated by BJ RAO

Corresponding editor: BJ RAO

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Roy, S., Bhattacharya, B., Bal, B. et al. A microscopic study on scattering in tissue section of Alternanthera philoxeroides under polarized light. J Biosci 46, 51 (2021). https://doi.org/10.1007/s12038-021-00174-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s12038-021-00174-2

Keywords

Search

Navigation