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Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties

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Abstract

Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. This tool has been successfully exploited for the selection of functional and specific binders in vitro. This review provides a comprehensive survey of the applications of ribosome display in screening or evolving functional proteins as well as in diagnostics and therapeutics. Previous papers on ribosome display failed to comprehensively review evolutionary strategies for proteins. In the present paper, we review all existing evolutionary strategies that have been combined with ribosome display. We also discuss shortcomings, improvement strategies, and research tendency.

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Abbreviations

E. coli :

Escherichia coli

scFv:

Single-chain variable fragment

SPR:

Surface plasmon resonance

RFs:

Release factors

RRFs:

Ribosome recycling factors

Cκ:

Constant region of Igκ chain

CH:

Heavy chain constant domain

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription-polymerase chain reaction

VHH:

Variable region of a heavy chain antibody

PM:

Parsimonious mutagenesis

RAGE:

Receptor for advanced glycation end products

DARPins:

Designed ankyrin repeat proteins

StEP:

Staggered extension process

IAA:

Indoleacetic acid

PrP:

Prion protein

BSA:

Bovine serum albumin

T-NHL:

T-cell non-Hodgkin lymphoma

DTT:

Dithiothreitol

HCV:

Hepatitis C virus

HCVcc:

HCV cell culture

PURE:

Protein synthesis using recombinant elements

ECD:

Extracellular domain

Her2:

Human epidermal growth factor receptor 2

K D :

Equilibrium dissociation constants

VH:

Heavy chain variable domain

K:

The complete constant region of the mouse κ light chain

pM:

Picomolar

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Acknowledgements

We would like to thank colleagues working on display technologies in our laboratory for their assistance in the field. This work is supported by Grants from National Natural Science Foundation of China (No. 31470967).

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Authors and Affiliations

  1. Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300350, People’s Republic of China

    Ruowei Li, Guangbo Kang, Min Hu & He Huang

  2. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People’s Republic of China

    Ruowei Li, Guangbo Kang, Min Hu & He Huang

  3. Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People’s Republic of China

    Ruowei Li, Guangbo Kang, Min Hu & He Huang

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  1. Ruowei Li
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Correspondence to He Huang.

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Li, R., Kang, G., Hu, M. et al. Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties. Mol Biotechnol 61, 60–71 (2019). https://doi.org/10.1007/s12033-018-0133-0

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