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A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice

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Abstract

Type 2C protein phosphatase plays an important role in the signal transduction of stress response in plants. In this paper, we identified a novel stress-induced type 2C protein phosphatase gene OsSIPP2C1 from rice. OsSIPP2C1 contains a complete open reading frame of 1,074 bp, encoding a protein with 357 amino acids. OsSIPP2C1 expression was up-regulated by high salt, PEG6000 and exogenous ABA, and enhanced in the abl1 mutant under normal, salt, or drought condition. Interestingly, OsSIPP2C1 expression was increased during the early panicle development. Subcellular localization assay using rice protoplast cells indicated that OsSIPP2C1 was predominantly located in the nucleus. Together, it is suggested that a nuclear PP2C protein OsSIPP2C1 negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.

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Acknowledgments

We thank Prof. Hongwei Xue from institute of plant physiology and ecology, SIBS, for kindly providing rice abl1 seeds. This work was supported by National Natural Science Foundation of China (nos. 30971556, 31071397), Jiangsu Agriculture Science and Technology Innovation Fund [no. CX(12)1003], State Key Laboratory of Rice Biology (no. 110101), and Fundamental Research Funds for the Central Universities (no. KYZ201137).

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Correspondence to Ji Huang.

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Yu-Sheng Li and Hui Sun contributed equally to this work.

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Li, YS., Sun, H., Wang, ZF. et al. A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice. Mol Biotechnol 54, 703–710 (2013). https://doi.org/10.1007/s12033-012-9614-8

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