Abstract
Adsorption of CSF Aβ1–42 during pre-analytical processing is suggested as an important confounder in testing. The aim of the present study was to assess the effect of polypropylene transfer plates (PTP) in the INNOTEST Aβ1–42 IVD-ELISA assay on Aβ1–42 levels. CSF samples from 26 individuals with subjective cognitive impairment (SCI) and 25 patients with suspected neurodegenerative disorders were tested using four different lots of kits. Aβ1–42 levels in all samples that were loaded onto the PTP were significantly lower than the levels in the same samples that were analyzed without prior loading onto the PTP. We found that the PTP may adsorb Aβ1–42 in the range 7 to 69%. The diagnosis in 20% of patients and amyloid burden assessment in 23% of SCI patients had to be modified post hoc due to initial erroneously low amyloid levels. Using a PTP prior to loading the samples onto the INNOTEST Aβ1–42 test plate may result in erroneously low Aβ1–42 levels.
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The English of the manuscript has been edited by EDANZ Language Editing Service with the reference number 17590.
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The present work was supported by the Scientific and Technological Research Council of Turkey-TUBITAK (Project No. 112S361) and by the Research Fund of Istanbul University (Project no: 27781).
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Erdinç Dursun: study concept and design, analysis and interpretation of data, study supervision, drafting and revision of manuscript for intellectual content
Merve Alaylıoğlu: study concept and design, acquisition and analysis of data
Başar Bilgiç: acquisition of data, revision of manuscript for intellectual content
Haşmet Hanağası: acquisition of data, revision of manuscript for intellectual content
Hakan Gürvit: acquisition of data, revision of manuscript for intellectual content
Murat Emre: acquisition of data, revision of manuscript for intellectual content
Duygu Gezen-Ak: study concept and design, acquisition, analysis, and interpretation of data, study supervision, drafting and revision of manuscript for intellectual content
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Dursun, E., Alaylıoğlu, M., Bilgiç, B. et al. Amyloid Beta Adsorption Problem with Transfer Plates in Amyloid Beta 1–42 IVD Kits. J Mol Neurosci 67, 534–539 (2019). https://doi.org/10.1007/s12031-019-1261-0
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DOI: https://doi.org/10.1007/s12031-019-1261-0