Abstract
Due to the lack of an appropriate animal model, few studies have addressed the integration of visual and vestibular information in the visual system. Using a mouse model with a visual defect (retinal degeneration fast, rdf), we have verified that the prepositus hypoglossal nucleus (PrH) and the Kooy cap of the inferior olive medial nucleus (IOK) are key regions in which visual and vestibular information integrate. Although the integration regions were identified, the precise mechanisms of integration require further investigation. The rdf mice and wild-type Kunming mice were randomly assigned to experimental and control subgroups, respectively. Mice in the experimental groups were exposed to rotary motion for 30 min three times at 24-h intervals, whereas mice in the control groups were not exposed to rotary motion. Differences in the number of calcitonin gene-related peptide positive (CGRP-positive) and choline acetyltransferase positive (ChAT-positive) neurons in the vestibular-related nucleus populations of two types of mice were determined. After rotatory stimulus, the number of CGRP-positive and ChAT-positive neurons in the PrH and the IOK was significantly less in rdf mice compared with that in wild-type mice. There were differences in the number of CGRP-positive and ChAT-positive neurons in the other vestibular-related regions, but the differences were not significant, except the difference in the number of ChAT-positive neurons in the medial vestibular nucleus. The expression patterns of CGRP and ChAT were similar to that of Fos in the vestibular-related regions in the two types of mice after rotatory stimulus. The number of CGRP-positive and ChAT-positive neurons and the number of active nerve cells were consistent in those regions in the two types of mice after rotary stimulus. Therefore, we speculated that CGRP and Ach generated and released by neurons in the PrH and the IOK may play roles in the sensory integration of visual and vestibular information in mice.
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References
Chi FL, Wang ZM, Li KY, Wu W (1999) Calcitonin gene-related peptide changes in efferent vestibular system during vestibular compensation. Chin J of Otorhinolaryngol 34(1):11–13
Franklin KB, Paxinos G (1997) The mouse brain in stereotaxic coordinates. Academic Press, San Diego, USA
Fu J, Sun Z, Yu L, Liu L (2003) Comparison of four stimulation patterns inducing motion sickness. J of Jilin Univ (Med Ed) 29(1):35–37
Goh J, Wiegmann D (2002) Human factors analysis of accidents involving visual flight rules flight into adverse weather. Aviat Space Environ Med 73(8):817–822
Golding JF (2006) Motion sickness susceptibility. Auton Neurosci 129(1–2):67–76
Holmes SR, Bunting A, Brown DL, Hiatt KL, Braithwaite MG, Harrigan MJ (2003) Survey of spatial disorientation in military pilots and navigators. Aviat Space Environ Med 74(9):957–965
Huang J, Stohl LL, Zhou X, Ding W, Granstein RD (2011) Calcitonin gene-related peptide inhibits chemokine production by human dermal microvascular endothelial cells. Brain Behav Immun 25(4):787–799
Jia HB, Xie SJ (2005) Advances in studies of spatial disorientation mechanism. Zhongguo Hangkong Hangtian Yixue Zazhi 16(4):305–308
Jiang ZL (2000) The pathogenesis and preventing and controlling measure of motion sickness. Jiaotong Yixue 14(5):433–435
Kaufman GD (2005) Fos expression in the vestibular brainstem: what one marker can tell us about the network. Brain Res Rev 50(1):200–211
Kong WJ, Scholtz AW, Hussl B, Kammen-Jolly K, Schrott-Fischer A (2002a) Localization of efferent neurotransmitters in the inner ear of the homozygous Bronx Waltzer mutant mouse. Hear Res 167(1–2):136–155
Kong WJ, Scholtz AW, Kammen-Jolly K, Glückert R, Hussl B, von Cauvenberg PB, Schrott-Fischer A (2002b) Ultrastructural evaluation of calcitonin gene-related peptide immunoreactivity in the human cochlea and vestibular endorgans. Eur J Neurosci 15(3):487–497
Long T (2005) The primary research on the retinal development of the mice with retinal degeneration. The Fourth Military Medical University, Xi’an
Mapp PI, McWilliams DF, Turley MJ, Hargin E, Walsh DA (2012) A role for the sensory neuropeptide calcitonin gene-related peptide in endothelial cell proliferation in vivo. Br J Pharmacol 10:1476–5381
Schrott-Fischer A, Kammen-Jolly K, Scholtz A, Rask-Andersen H, Glueckert R, Eybalin M (2007) Efferent neurotransmitters in the human cochlea and vestibule. Acta Otolaryngol 127(1):13–19
Shinder ME, Perachio AA, Kaufman GD (2005a) Fos responses to short-term adaptation of the horizontal vestibuloocular reflex before and after vestibular compensation in the Mongolian gerbil. Brain Res 1050(1–2):79–93
Shinder ME, Perachio AA, Kaufman GD (2005b) VOR and Fos response during acute vestibular compensation in the Mongolian gerbil in darkness and in light. Brain Res 1038(2):183–197
Vause CV, Durham PL (2010) Calcitonin gene-related peptide differentially regulates gene and protein expression in trigeminal glia cells: findings from array analysis. Neurosci Lett 473(3):163–167
Wackym PA, Popper P, Ward PH, Micevych PE (1991) Cell and molecular anatomy of nicotinic acetylcholine receptor subunits and calcitonin gene-related peptide in the rat vestibular system. Otolaryngology-head and Neck Surg 105(4):493–510
Wang XC (2006) The primary research on the role of calcitonin gene-related peptide on the pathogenesis of motion sickness in rats. The Fourth Military Medical University, Xi’an
Wang XC, Feng LN, Zhang ZM (2006) Effect of rotatory stimulation on plasma level of calcitonin gene related peptide in rats. Disi Junyi Daxue Xuebao 27(22):2020–2022
Wang XC, Feng LN, Zhang ZM (2010) Effect of rotary motion on Fos protein expression in the vestibular-related nucleus population in a mouse model of rapid retinal degeneration. Neural Regen Res 5(7):555–560
Xiaocheng W, Zhaohui S, Junhui X, Lei Z, Lining F et al (2012) Expression of calcitonin gene-related peptide in efferent vestibular system and vestibular nucleus in rats with motion sickness. PLoS ONE 7(10):e47308
Zajonc TP, Roland PS (2006) Vertigo and motion sickness. Part II: pharmacologic treatment. Ear Nose Throat J 85(1):25–35
Zefeng W, Ping J, Xinkang T, Ye Z (2008) Localization of choline aceyltransferase during ante partum and postnatal development in the rat brain. Chin J of Neuroanat 24(2):176–180
Zhen ZHAO, Wen-juan MI, Shun-li LIU, Jian-hua QIU (2008) The distribution of TH and ChAT positive neurons in the facial nucleus of rats and its implication. Chin J of Otol 6(3):273–277
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Wang Xiaocheng, Shi Zhaohui, and Bian Ka contributed equally to this work.
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Xiaocheng, W., Zhaohui, S., Ka, B. et al. The Expression of Calcitonin Gene-Related Peptide and Acetylcholine in the Vestibular-Related Nucleus Population of Wild-Type Mice and Retinal Degeneration Fast Mice After Rotary Stimulation. J Mol Neurosci 51, 514–521 (2013). https://doi.org/10.1007/s12031-013-0087-4
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DOI: https://doi.org/10.1007/s12031-013-0087-4