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Role of DIDO1 in Progression of Esophageal Squamous Cell Carcinoma

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Journal of Gastrointestinal Cancer Aims and scope Submit manuscript

Abstract

Purpose

Apoptosis is one of the main involved processes during development and organogenesis and its aberration may result in tumorigenesis. In the present study, due to the role of death inducer-obliterator 1 (DIDO1) in activation of caspases 9 and 3 during the apoptosis process, the role of DIDO1 as the shortest splicing variant of the DIDO gene was assessed for the first time in esophageal squamous cell carcinoma (ESCC) patients.

Methods

DIDO1 mRNA expression in tumor tissues from 50 ESCC patients was compared to their corresponding margin normal tissues using the real-time polymerase chain reaction (RT-PCR).

Results

Nine out of 50 (18%) and 13 out of 50 (26%) cases had DIDO1 under- and overexpression, respectively. There was a significant correlation between DIDO1 mRNA expression and tumor depth of invasion (p = 0.050). Also, there was a significant correlation between age of patients and levels of DIDO1 mRNA expression (p = 0.039).

Conclusions

This study is the first report that assessed the DIDO1 expression in ESCC patients and revealed its probable role in the early steps of tumor progression and metastasis. Therefore, DIDO1 can be suggested as a marker for the primary ESCCs.

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References

  1. Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):115–32. https://doi.org/10.3322/caac.21338.

    Article  PubMed  Google Scholar 

  2. Wei WQ, Chen ZF, He YT, Feng H, Hou J, Lin DM, et al. Long-term follow-up of a community assignment, one-time endoscopic screening study of esophageal cancer in China. J Clin Oncol. 2015;33(17):1951–7. https://doi.org/10.1200/JCO.2014.58.0423.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Kamangar F, Dores GM, Anderson WF. Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 2006;24(14):2137–50. https://doi.org/10.1200/JCO.2005.05.2308.

    Article  PubMed  Google Scholar 

  4. Jacobson MD, Weil M, Raff MC. Programmed cell death in animal development. Cell. 1997;88(3):347–54.

    Article  CAS  PubMed  Google Scholar 

  5. Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972;26(4):239–57.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Raff M. Cell suicide for beginners. Nature. 1998;396(6707):119–22. https://doi.org/10.1038/24055.

    Article  CAS  PubMed  Google Scholar 

  7. Ellis RE, Yuan JY, Horvitz HR. Mechanisms and functions of cell death. Annu Rev Cell Biol. 1991;7:663–98. https://doi.org/10.1146/annurev.cb.07.110191.003311.

    Article  CAS  PubMed  Google Scholar 

  8. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998;281(5381):1312–6.

    Article  CAS  PubMed  Google Scholar 

  9. Budihardjo I, Oliver H, Lutter M, Luo X, Wang X. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 1999;15:269–90. https://doi.org/10.1146/annurev.cellbio.15.1.269.

    Article  CAS  PubMed  Google Scholar 

  10. Earnshaw WC, Martins LM, Kaufmann SH. Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem. 1999;68:383–424. https://doi.org/10.1146/annurev.biochem.68.1.383.

    Article  CAS  PubMed  Google Scholar 

  11. Salvesen GS, Dixit VM. Caspases: intracellular signaling by proteolysis. Cell. 1997;91(4):443–6.

    Article  CAS  PubMed  Google Scholar 

  12. Wagner AJ, Kokontis JM, Hay N. Myc-mediated apoptosis requires wild-type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21waf1/cip1. Genes Dev. 1994;8(23):2817–30.

    Article  CAS  PubMed  Google Scholar 

  13. Kumar A, Commane M, Flickinger TW, Horvath CM, Stark GR. Defective TNF-alpha-induced apoptosis in STAT1-null cells due to low constitutive levels of caspases. Science. 1997;278(5343):1630–2.

    Article  CAS  PubMed  Google Scholar 

  14. Evan GI, Wyllie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, et al. Induction of apoptosis in fibroblasts by c-myc protein. Cell. 1992;69(1):119–28.

    Article  CAS  PubMed  Google Scholar 

  15. Ham J, Babij C, Whitfield J, Pfarr CM, Lallemand D, Yaniv M, et al. A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death. Neuron. 1995;14(5):927–39.

    Article  CAS  PubMed  Google Scholar 

  16. Baichwal VR, Baeuerle PA. Activate NF-kappa B or die? Curr Biol. 1997;7(2):R94–6.

    Article  CAS  PubMed  Google Scholar 

  17. Futterer A, Campanero MR, Leonardo E, Criado LM, Flores JM, Hernandez JM, et al. Dido gene expression alterations are implicated in the induction of hematological myeloid neoplasms. J Clin Invest. 2005;115(9):2351–62. https://doi.org/10.1172/JCI24177.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Futterer A, Raya A, Llorente M, Izpisua-Belmonte JC, de la Pompa JL, Klatt P, et al. Ablation of Dido3 compromises lineage commitment of stem cells in vitro and during early embryonic development. Cell Death Differ. 2012;19(1):132–43. https://doi.org/10.1038/cdd.2011.62.

    Article  CAS  PubMed  Google Scholar 

  19. Garcia-Domingo D, Ramirez D, Gonzalez de Buitrago G, Martinez AC. Death inducer-obliterator 1 triggers apoptosis after nuclear translocation and caspase upregulation. Mol Cell Biol. 2003;23(9):3216–25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Gatchalian J, Futterer A, Rothbart SB, Tong Q, Rincon-Arano H, Sanchez de Diego A, et al. Dido3 PHD modulates cell differentiation and division. Cell Rep. 2013;4(1):148–58. https://doi.org/10.1016/j.celrep.2013.06.014.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Garcia-Domingo D, Leonardo E, Grandien A, Martinez P, Albar JP, Izpisua-Belmonte JC, et al. DIO-1 is a gene involved in onset of apoptosis in vitro, whose misexpression disrupts limb development. Proc Natl Acad Sci U S A. 1999;96(14):7992–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Moghbeli M, Forghanifard MM, Aarabi A, Mansourian A, Abbaszadegan MR. Clinicopathological sex-related relevance of musashi1 mRNA expression in esophageal squamous cell carcinoma patients. Pathol Oncol Res. 2014;20(2):427–33. https://doi.org/10.1007/s12253-013-9712-3.

    Article  CAS  PubMed  Google Scholar 

  23. Raeisossadati R, Farshchian M, Ganji A, Tavassoli A, Velayati A, Dadkhah E, et al. Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients. Int J Color Dis. 2011;26(10):1265–70. https://doi.org/10.1007/s00384-011-1230-8.

    Article  Google Scholar 

  24. Trachana V, van Wely KH, Guerrero AA, Futterer A, Martinez AC. Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability. Proc Natl Acad Sci U S A. 2007;104(8):2691–6. https://doi.org/10.1073/pnas.0611132104.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Rojas AM, Sanchez-Pulido L, Futterer A, van Wely KH, Martinez AC, Valencia A. Death inducer obliterator protein 1 in the context of DNA regulation. Sequence analyses of distant homologues point to a novel functional role. FEBS J. 2005;272(14):3505–11. https://doi.org/10.1111/j.1742-4658.2005.04759.x.

    Article  CAS  PubMed  Google Scholar 

  26. Wang Y, Chen Y, Chen Z, Wu Q, Ke WJ, Wu QL. Gambogic acid induces death inducer-obliterator 1-mediated apoptosis in Jurkat T cells. Acta Pharmacol Sin. 2008;29(3):349–54. https://doi.org/10.1111/j.1745-7254.2008.00762.x.

    Article  CAS  PubMed  Google Scholar 

  27. Prieto I, Kouznetsova A, Futterer A, Trachana V, Leonardo E, Alonso Guerrero A, et al. Synaptonemal complex assembly and H3K4Me3 demethylation determine DIDO3 localization in meiosis. Chromosoma. 2009;118(5):617–32. https://doi.org/10.1007/s00412-009-0223-7.

    Article  CAS  PubMed  Google Scholar 

  28. Santiveri CM, Garcia-Mayoral MF, Perez-Canadillas JM, Jimenez MA. NMR structure note: PHD domain from death inducer obliterator protein and its interaction with H3K4me3. J Biomol NMR. 2013;56(2):183–90. https://doi.org/10.1007/s10858-013-9726-x.

    Article  CAS  PubMed  Google Scholar 

  29. Lee JH, Hart SR, Skalnik DG. Histone deacetylase activity is required for embryonic stem cell differentiation. Genesis. 2004;38(1):32–8. https://doi.org/10.1002/gene.10250.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

    Mohammad Mahdi Forghanifard

  2. Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Pegah Naeimi Khorasanizadeh, Mohammad Reza Abbaszadegan & Afsaneh Javdani Mallak

  3. Department of Modern Sciences and Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Meysam Moghbeli

Authors
  1. Mohammad Mahdi Forghanifard
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  2. Pegah Naeimi Khorasanizadeh
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  3. Mohammad Reza Abbaszadegan
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Correspondence to Meysam Moghbeli.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Forghanifard, M.M., Naeimi Khorasanizadeh, P., Abbaszadegan, M.R. et al. Role of DIDO1 in Progression of Esophageal Squamous Cell Carcinoma. J Gastrointest Canc 51, 83–87 (2020). https://doi.org/10.1007/s12029-019-00212-1

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  • DOI: https://doi.org/10.1007/s12029-019-00212-1

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