Abstract
In this study, we compare digestive methods used in a forensic context to extract diatoms (37 % hydrochloric acid) to a method recently described in Italian protocols for analysis of benthic diatoms for ecological assessment of surface water (hydrogen peroxide digestion). The two digestive methods were performed using 5 g of brain, lung, liver, kidney, and bone marrow taken from the bodies of 10 drowning victims recovered from three different aquatic environments (ocean, lakes, and rivers). Postmortem examination was performed on all bodies, but aquatic samples were only analyzed in two cases. Tissue digestion was equal by both methods. Hydrogen peroxide (H2O2) digestion resulted in better diatom preservation, enabling identification of nine genera in all samples examined versus three obtained using hydrochloride digestion. The ideal digestive method to provide evidence for corroboration of a diagnosis of drowning still needs to be established. However, the benthic diatoms protocol can be useful because it is less chemically hazardous to the laboratory operator and supports better diatom preservation for reliable taxonomic analysis.
References
DiMaio DJ, DiMaio VJM. Drowning. In: DiMaio DJ, DiMaio VJM, editors. Forensic pathology. Amsterdam: Elsevier; 1989. p. 357–65.
Piette MHA, De Letter EA. Drowning: still a difficult autopsy diagnosis. Forensic Sci Int. 2006;163:1–9.
Ludes B, Fornes P. Drowing. In: Payne-James J, Basuttil A, Smock W, editors. Forensic medicine: clinical and pathologycal aspects. Grenwich: Medical Media; 2003. p. 247–57.
Lunetta P, Modell JH. Macroscopical, microscopical, and laboratory findings in drowning victims. In: Tsokos M, editor. Forensic pathology reviews, vol. 3. Totowa: Humana Press; 2005. p. 3–56.
Saukko P, Knight B. Knight’s forensic pathology. 3rd ed. London: Arnold; 2004. p. 406–8.
Spitz WU. Investigation of bodies in water. In: Spitz WU, editor. Medicolegal investigation of death. 4th ed. Springfield: Charles C Thomas; 2004. p. 846–81.
Farrugia A, Ludes B. Diagnostic of drowning in forensic medicine. In: Vieira DN, editor. Forensic medicine—from old problems to new challenges. InTech: Rijeka; 2011. p. 59–60.
Der Revenstorf V. Nachweis der aspirirtenetrankungsflussigkeitalskriterium des todesdurchetrinken, vereteljahresschs. Geritchtl Med Off Sanitaetswes. 1904;28:274–9.
Ludes B, Coste M, North N, Doray S, Tracqui A, Kintz P. Diatoms analysis. Int J Legal Med. 1999;112:163–6.
Hurlimann J, Feer P, Elber F, Niederberger K, Dirnofer R, Wyler D. Diatom detection in the diagnosis of death by drowning. Int J Legal Med. 2000;114:6–14.
Auer A, Mottonem M. Diatoms and drowning. Z Rechtsmed. 1988;101:87–98.
Kobayashi M, Yamada Y, Zhang W-D, Ttakura Y, Nagao M, Takatori T. Novel detection of plankton from lung tissue by enzymatic digestion method. Forensic Sci Int. 1993;60:81–90.
Sidari L, Nunno ND, Costantinides F, Melato M. Diatom test with Soluene 350 to diagnose drowning in seawater. Forensic Sci Int. 1999;103:61–5.
Council of European Communities Directive 2000/60/EEC of 23 October 2000 establishing a framework for community action in the field of water policy. Luxembourg. Official Journal of European Communities. http://www.direttivaacque.minambiente.it/
EN 13946. Water quality. Guidance Standard for the routine sampling and pre-treatment of benthic diatom samples from rivers, European Committee for Standardization, Brussels. 2003. http://www.safrass.com/partners_area/BSI%20Benthic%20diatoms.pdf
ISPRA Metodi biologici per le acque—Parte I. Manuali e Linee Guida APAT. 2007. http://www.isprambiente.gov.it/it/pubblicazioni/manuali-e-linee-guida/metodi-biologici-per-le-acque-parte-i
ISPRA. Metodi Biologici per le acque superficiali interne. Manuali e Linee guida 111/2014. www.isprambiente.gov.it/…/MLG__111_2014_Metodi_Biologici_acque
Dell’Uomo A. L’Indice Diatomico di Eutrofizzazione/Polluzione (EPI-D) nel monitoraggio delle acque correnti. 2004 Linee Guida. APAT, CTN AIM. 101. http://www.cisba.eu/temi/18-fiumi/75-epi-d
Jeffrey SW, Hallegraeff GM. Phytoplankton ecology of Australasian waters. In: Clayton MN, King RJ, editors. Biology of marine plants. Australia: Longman; 1990. p. 310–48.
Mancini L, Sollazzo C. Metodo per la valutazione dello stato ecologico delle acque correnti: comunità diatomiche. Rapporto ISTISAN 09/19 2009: Roma: Istituto Superiore di Sanità. http://www.iss.it/binary/publ/cont/0919web.pdf
Deny L. Relation of abundance-weighted averages of diatom indicator values to measured environmental conditions in standing freshwaters. Ecol Ind. 2004;4:225–75.
Recommendation No. R (99) 3 of the Committee of Ministers to member states on the harmonization of medico-legal autopsy rules. Forensic Sci Int. 2000;111(1–3):5–58.
Auer A. Qualitative diatom analysis as a tool to diagnose drowning. Am J Forensic Med Pathol. 1991;12:213–8.
Ago K, Hayashi T, Ago M, Ogata M. The number of diatoms recovered from the lungs and other organs in drowning deaths in bathwater. Legal Med. 2011;13:186–90.
Lunetta P, Miettinen A, Spilling K, Sajantila A. False positive diatom test: a real challenge? A post mortem study using standardized protocol. Legal Med. 2013;15:229–34.
Kahlert M, Kelly M, Albert RL, Almeida SFP, Saul B, et al. Identification versus counting protocols as sources of uncertainty in diatom-based ecological status assessments. Hydrobiologia. 2012;695:109–24.
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An erratum to this article is available at http://dx.doi.org/10.1007/s12024-016-9833-6.
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Fucci, N., Pascali, V.L., Puccinelli, C. et al. Evaluation of two methods for the use of diatoms in drowning cases. Forensic Sci Med Pathol 11, 601–605 (2015). https://doi.org/10.1007/s12024-015-9708-2
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DOI: https://doi.org/10.1007/s12024-015-9708-2