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Preventive Effects of Sinigrin Against the Memory Deterioration in the Pentylenetetrazole-Kindled Male Wistar Rats: Possible Modulation of NLRP3 Pathway

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Abstract

Mainly found in brussels sprouts, broccoli, and black mustard seeds, sinigrin (2-propenyl glucosinolate) has enjoyed some attention currently for its effects on health and disease prevention. The present research design is aimed at investigating the effects of sinigrin on inflammation, oxidative stress (OS) and memory. Randomly, six groups of male Wistar rats were categorized into the control and experimental groups. The experimental groups were treated with sinigrin (10 and 20 mg/kg, orally). The control positive group was given the pentylenetetrazole (PTZ) treatment and the control negative one was given normal saline. All groups were kindled by the sub-threshold dose (35 mg/kg, i.p.) of PTZ for 12 times in one month. When the kindling procedure was done, the seizure behaviors and the behavioral function were evaluated. For cognitive parameters, the shuttle box test was employed. When the experiment was terminated, the rats were euthanized and their blood serum as well as brain samples were isolated for respective measuring of OS and gene expression parameters. The treatment with sinigrin significantly delayed the appearance of the seizure symptoms in comparison to that of the PTZ group. It also significantly increased the memory parameters like retention latency and the total time having been spent in the light compartment in the epileptic rats. In addition, sinigrin increased the superoxide dismutase and catalase levels. Treatment with sinigrin suppressed the Il1b and Nlrp3 gene expression at hippocampal level. In sum, sinigrin prevents inflammation, OS and memory impairment against the PTZ-kindling epilepsy in rats.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    Fatemeh Aghaie & Abdolkarim Hosseini

  2. Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Mojgan Rajabi

  3. Laboratory Center, Emam Reza Hospital, Islamshahr, Iran

    Amin Hosseini

  4. Department of Biology, Women’s University of Semnan, Semnan, Iran

    Fatemeh Moradifar

  5. Department of Exercise Physiology, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran

    Samaneh Koneshlou

Authors
  1. Fatemeh Aghaie
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  2. Mojgan Rajabi
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  3. Amin Hosseini
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  4. Fatemeh Moradifar
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  5. Samaneh Koneshlou
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  6. Abdolkarim Hosseini
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Contributions

Conception and design of the work: FA, MR, AH, FM, SK, AH; data collection: FA, MR, AH, FM, SK, AH; analysis and interpretation of the data: FA, MR, AH, FM, SK, AH; statistical analysis: FA, MR, AH, FM, SK, AH; drafting the manuscript: FA, MR, AH, FM, SK, AH; critical revision of the manuscript: FA, MR, AH, FM, SK, AH.

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Correspondence to Abdolkarim Hosseini.

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All experiments implemented in the present study observed standard ethical guidelines stipulated by European Communities Directive (2010/63/EU); they also had the approval of Shahid Beheshti University’s Local Ethics Committee (# IR.SBU.REC.1399.085).

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Aghaie, F., Rajabi, M., Hosseini, A. et al. Preventive Effects of Sinigrin Against the Memory Deterioration in the Pentylenetetrazole-Kindled Male Wistar Rats: Possible Modulation of NLRP3 Pathway. Neuromol Med 24, 311–319 (2022). https://doi.org/10.1007/s12017-021-08690-y

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  • DOI: https://doi.org/10.1007/s12017-021-08690-y

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