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Temporal and Spatial Expression of Cyclin H in Rat Spinal Cord Injury

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Abstract

Cyclin H regulates cell cycle transitions; it always forms trimeric cyclin-dependent protein kinases (CDK)-activating kinase (CAK) complex with CDK7 and MAT1 that phosphorylates a threonine residue in the CDK2 T loop region. However, neither the expression nor function of cyclin H in the central nervous system (CNS) injury is still clear. Therefore, we studied cyclin H in a rat spinal cord contusion model. Injury markedly increased cyclin H protein expression throughout the thoracic spinal cord but did not increase CDK7. However, double immunofluorescent staining for proliferating cell nuclear antigen (PCNA) and cell markers revealed increases of cyclin H and CDK2 in proliferating microglia and astrocytes, and the co-immunoprecipitation studies shown that the associations of cyclin H with CDK2 were enhanced evidently after injury. Our data suggest that cyclin H may play a proliferative role in spinal cord injury (SCI).

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Abbreviations

BSA:

Bovine serum albumin

CAK:

CDK-activating kinase

CDKs:

Cyclin-dependent protein kinases

CNS:

Central nervous system

DAB:

Diaminobenzidin

ECL:

Enhanced chemiluminescence system

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GFAP:

Glial fibrillary acidic protein

PAGE:

Polyacrylamide gel electrophoresis

PCNA:

Proliferating cell nuclear antigen

SCI:

Spinal cord injury

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.30671046, No.30770488, No.30870320, No.31070723, No.31071288 and No.81070275); Natural Science Foundation of Jiangsu province (No. BK2009156, No. BK2009157 and No. BK2009161); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Hospital of Nantong University, and The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, People’s Republic of China

    Gang Wu, Jianhua Cao, Chen Peng, Huiguang Yang, Zhiming Cui, Jian Zhao, Qiyun Wu, Honghui Li, Xingxing Gu & Feng Zhang

  2. Department of Neurosurgery, Affiliated HaiKou Hospital of Xiangya School of Medicine, Central South University, Hai kou, 570208, People’s Republic of China

    Jiangli Han

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  1. Gang Wu
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Correspondence to Xingxing Gu or Feng Zhang.

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Gang Wu and Jianhua Cao contributed equally to this work.

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Wu, G., Cao, J., Peng, C. et al. Temporal and Spatial Expression of Cyclin H in Rat Spinal Cord Injury. Neuromol Med 13, 187–196 (2011). https://doi.org/10.1007/s12017-011-8150-1

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  • DOI: https://doi.org/10.1007/s12017-011-8150-1

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