Abstract
Cadmium (Cd) is a heavy metal in natural environment and has extreme toxicity. Selenium (Se) has protective effect against heavy metal-induced injury or oxidative stress. Cytochrome P450 (CYP450) enzymes are a family of hemoproteins primarily responsible for detoxification functions. In order to investigate whether CYP450 is related to the damage of livers caused by Cd exposure, we chose forty-eight 28-day-old healthy Hailan cocks for four groups: control group, Se group, Cd group, and Se + Cd group. After 90-day treatment, euthanized for experiment. Based on an established subchronic Cd poisoning model in chicken, this experiment was designed to detect mitochondrial structure, malondialdehyde (MDA), glutathione (GSH), DNA and protein crosslink (DPC) and protein carbonyl (PCO) content, the CYP450 and b5 contents, the aminopyrine-N-demethylase (AND), erythromycin N-demethylase (ERND), aniline 4-hydroxylase (AH) and NADPH-cytochrome C reducatase (CR) activities, and mRNA expression level in the livers. The present results indicated that the MDA content, PCO content, and DPC index in Cd group were higher than those observed in other three groups. Most of the mitochondrial structure is incomplete in Cd group. The contents of CYP450 and b5 were decreased in Cd group. The activities of AND, ERND, AH, and CR got reduced after Cd exposure, as observed in CYP450 gene expression. Our results showed that CYP450 system was involved in the entire process of injury and protection. This research provides a comprehensive evaluation of the oxidative stress effects of Cd related to CYP450 in chicken.
Similar content being viewed by others
References
Dorian C, Gattone VH 2nd, Klaasen CD (1992) Renal cadmium deposition and injury as a result of accumulation of cadmium-metallothionein (CdMT) by the proximal convoluted tubules—a light microscopic autoradiography study with 109CdMT. Toxicol Appl Pharmacol 114:173–181
Chen M, Li X, Fan R, Yang J, Jin X, Hamid S, Xu S (2017) Cadmium induces BNIP3 -dependent autophagy in chicken spleen by modulating miR-33-AMPK axis. Chemosphere 194:396–402
Yamano T, DeCicco LA, Rikans LE (2000) Attenuation of cadmium-induced liver injury in senescent male fischer 344 rats: role of Kupffer cells and inflammatory cytokines. Toxicol Appl Pharmacol 162:68–75
Zhang Z, Zheng Z, Cai J, Liu Q, Yang J, Gong Y, Wu M, Shen Q, Xu S (2017) Effect of cadmium on oxidative stress and immune function of common carp (Cyprinus carpio L.) by transcriptome analysis. Aquat Toxicol 192:171–177
Pathak N, Khandelwal S (2006) Oxidative stress and apoptotic changes in murine splenocytes exposed to cadmium. Toxicology 220:26–36
Zhou YJ, Zhang SP, Liu CW, Ca i YQ (2009) The protection of selenium on ROS mediated-apoptosis by mitochondria dysfunction in cadmium-induced LLC-PK(1) cells. Toxicol in Vitro 23:288–294
Chu BX, Fan RF, Lin SQ, Yang DB, Wang ZY, Wang L (2018) Interplay between autophagy and apoptosis in lead (II)-induced cytotoxicity of primary rat proximal tubular cells. J Inorg Biochem 182:184–193
Wang Y, Zhao H, Liu J, Shao Y, Li J, Luo L, Xing M (2018) Copper and arsenic-induced oxidative stress and immune imbalance are associated with activation of heat shock proteins in chicken intestines. Int Immunopharmacol 60:64–75
Wang Y, Zhao H, Shao Y, Liu J, Li J, Luo L, Xing M (2018) Copper (II) and/or arsenite-induced oxidative stress cascades apoptosis and autophagy in the skeletal muscles of chicken. Chemosphere 206:597–605
Song XB, Liu G, Liu F, Yan ZG, Wang ZY, Liu ZP, Wang L (2017) Autophagy blockade and lysosomal membrane permeabilization contribute to lead-induced nephrotoxicity in primary rat proximal tubular cells. Cell Death Dis 8:e2863
Guengerich FP (2003) Cytochromes P450, drugs, and diseases. Mol Interv 3:194–204
Guengerich FP (2001) Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. Chem Res Toxicol 14(6):611–650
Dong X, Zhu L, Wang J, Wang J, Xie H, Hou X, Jia W (2009) Effects of atrazine on cytochrome P450 enzymes of zebrafish (Danio rerio). Chemosphere 77:404–412
Lu X, Li Y, Thunders M, Cavanagh J, Matthew C, Wang X, Zhou X, Qiu J (2017) Differential protein expression and localization of CYP450 enzymes in three species of earthworm; is this a reflection of environmental adaptation? Chemosphere 171:485–490
Baker JR, Satarug S, Reilly PE, Edwards RJ, Ariyoshi N, Kamataki T, Moore MR, Williams DJ (2001) Relationships between non-occupational cadmium exposure and expression of nine cytochrome P450 forms in human liver and kidney cortex samples. Biochem Pharmacol 62:713–721
Reed JR, Cawley GF, Ardoin TG, Dellinger B, Lomnicki SM, Hasan F, Kiruri LW, Backes WL (2014) Environmentally persistent free radicals inhibit cytochrome P450 activity in rat liver microsomes. Toxicol Appl Pharmacol 277(2):200–209
Mi Y, Li J, Lin X, Liu X, Zhang C (2018) Grape seed Proanthocyanidin extract prevents ovarian aging by inhibiting oxidative stress in the hens. Oxidative Med Cell Longev:1–16
Tobiasz A, Walas S, Filek M, Mrowiec H, Samsel K, Sieprawska A, Hartikainen H (2014) Effect of selenium on distribution of macro- and micro-elements to different tissues during wheat ontogeny. Biol Plant 58:370–374
Xi J, Zhe X, Xia Z, Chen M, Xu S (2017) The antagonistic effect of selenium on lead-induced apoptosis via mitochondrial dynamics pathway in the chicken kidney. Chemosphere 180:259–266
Yao H, Liu W, Zhao W, Fan R, Zhao X, Khoso PA, Zhang Z, Xu S (2014) Different responses of selenoproteins to the altered expression of selenoprotein W in chicken myoblasts. RSC Adv 4:64032–64042
Yao HD, Wu Q, Zhang ZW, Li S, Wang XL, Lei XG, Xu SW (2013) Selenoprotein W serves as an antioxidant in chicken myoblasts. Biochim Biophys Acta 1830:3112–3120
Li X, Xing M, Chen M, Zhao J, Fan R, Zhao X, Cao C, Yang J, Zhang Z, Xu S (2017) Effects of selenium-lead interaction on the gene expression of inflammatory factors and selenoproteins in chicken neutrophils. Ecotoxicol & Environ Saf 139:447–453
Yao HD, Wu Q, Zhang ZW, Zhang JL, Li S, Huang JQ, Ren FZ, Xu SW, Wang XL, Lei XG (2013) Gene expression of endoplasmic reticulum resident selenoproteins correlates with apoptosis in various muscles of se-deficient chicks. J Nutr 143:613–619
Chen X, Zhu YH, Cheng XY, Zhang ZW, Xu SW (2012) The protection of selenium against cadmium-induced cytotoxicity via the heat shock protein pathway in chicken splenic lymphocytes. Molecules 17:14565–14572
Erkekoglu P, Giray BK, Caglayan A, Hincal F (2012) Selenium and/or iodine deficiency alters hepatic xenobiotic metabolizing enzyme activities in rats. J Trace Elem Med Biol 26:36–41
Zhu WJ, Zhang ZW, Wang XS, Xu SW, Li M, Li S (2014) Effects of avermectin on microsomal cytochrome P450 enzymes in the liver and kidneys of pigeons. Environ Toxicol Pharmacol 38:562–569
Dupont I, Berthou F, Bodenez P, Bardou L, Guirriec C, Stephan N, Dreano Y, Lucas D (1999) Involvement of cytochromes P-450 2E1 and 3A4 in the 5-hydroxylation of salicylate in humans. Drug Metab Dispos 27:322
Brattsten LB, Price SL, Gunderson CA (1980) Microsomal oxidases in midgut and fatbody tissues of a broadly herbivorous insect larva, Spodoptera eridania cramer (Noctuidae). Comp Biochem Physiol C: Comparative Pharmacology 66:231–237
Fu Y, Li M, Liu C, Qu JP, Zhu WJ, Xing HJ, Xu SW, Li S (2013) Effect of atrazine and chlorpyrifos exposure on cytochrome P450 contents and enzyme activities in common carp gills. Ecotoxicol Environ Saf 94:28–36
Yang DB, Yang H, Wang L, Wang MG, Wang XY, Wang ZY (2017) Trehalose protects against cadmium-induced cytotoxicity in primary rat proximal tubular cells via inhibiting apoptosis and restoring autophagic flux. Cell Death Dis 8:e3099
Liu L, Yang B, Cheng Y, Lin H (2015) Ameliorative effects of selenium on cadmium-induced oxidative stress and endoplasmic reticulum stress in the chicken kidney. Biol Trace Elem Res 167:308–319
Li JL, Jiang CY, Li S, Xu SW (2013) Cadmium induced hepatotoxicity in chickens (Gallus domesticus) and ameliorative effect by selenium. Ecotoxicol Environ Saf 96:103–109
Yang S, Zhang Z, He J, Li J, Zhang J, Xing H, Xu S (2012) Ovarian toxicity induced by dietary cadmium in hen. Biol Trace Elem Res 148:53–60
Tretyakova NY, Th GA, Ji S (2015) DNA-protein cross-links: formation, structural identities, and biological outcomes. Acc Chem Res 48:1631–1644
Tejaswi G, Suchitra MM, Rajasekhar D, Kiranmayi VS, Rao PVLNS (2017) Myeloperoxidase, protein carbonyls and oxidative stress in coronary artery disease. J Indian Coll Cardiol 7:149–152
Tsikas D (2016) Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem 524:13–30
Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ (2003) Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem 278:31426–31433
Schwedhelm E, Benndorf RA, Boger RH, Tsikas D (2007) Mass spectrometric analysis of F2-Isoprostanes: markers and mediators in human disease. Curr Pharm Anal 3:39–51
Prieto I, Monsalve M (2017) ROS homeostasis, a key determinant in liver ischemic-preconditioning. Redox Biol 12:1020–1025
Groehler TA, Kren S, Li Q, Robledovillafane M, Schmidt J, Garry M, Tretyakova N (2018) Oxidative cross-linking of proteins to DNA following ischemia-reperfusion injury. Free Radic Biol Med 120:89–101
Nag T, Ghosh A (2013) Cardiovascular disease risk factors in Asian Indian population: a systematic review. J Cardiovasc Dis Res 4:222–228
Garibaldi S, Aragno I, Odetti P, Marinari UM (1994) Relationships between protein carbonyls, retinol and tocopherols level in human plasma. Biochem Mol Biol Int 34:729–736
Zhao P, Guo Y, Zhang W, Chai H, Xing H, Xing M (2016) Neurotoxicity induced by arsenic in Gallus Gallus: regulation of oxidative stress and heat shock protein response. Chemosphere 166:238–245
Sereemaspun A, Hongpiticharoen P, Rojanathanes R, Maneewattanapinyo P (2008) Inhibition of human cytochrome P450 enzymes by metallic nanoparticles: a preliminary to Nanogenomics. Int J Pharmacol 4:492–495
Fröhlich E, Kueznik T, Samberger C, Roblegg E, Wrighton C, Pieber TR (2010) Size-dependent effects of nanoparticles on the activity of cytochrome P450 isoenzymes. Toxicol Appl Pharmacol 242:326–332
Rayman MP, Lyons TP, Cole DJA (2000) The importance of selenium to human health. Lancet 356:233–241
Yang J, Zhang Y, Hamid S, Cai J, Liu Q, Li H, Zhao R, Wang H, Xu S, Zhang Z (2017) Interplay between autophagy and apoptosis in selenium deficient cardiomyocytes in chicken. J Inorg Biochem 170:17–25
Liu T, Yang T, Xu Z, Tan S, Pan T, Wan N, Li S (2018) MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1. J Inorg Biochem 186:235–245
Karabulut-Bulan O, Bolkent S, Yanardag R, Bilgin-Sokmen B (2008) The role of vitamin C, vitamin E, and selenium on cadmium-induced renal toxicity of rats. Drug Chem Toxicol 31:413–426
Wang W, Chen M, Jin X, Li X, Yang Z, Lin H, Xu S (2018) H2S induces Th1/Th2 imbalance with triggered NF-κB pathway to exacerbate LPS-induce chicken pneumonia response. Chemosphere 208:241–246
Jin X, Jia T, Liu R, Xu S (2018) The antagonistic effect of selenium on cadmium-induced apoptosis via PPAR-γ/PI3K/Akt pathway in chicken pancreas. J Hazard Mater 357:355–362
Li JL, Gao R, Li S, Wang JT, Tang ZX, Xu SW (2010) Testicular toxicity induced by dietary cadmium in cocks and ameliorative effect by selenium. Biometals 23:695–705
Bansal MP, Kaur P (2005) Selenium, a versatile trace element: current research implications. Indian J Exp Biol 43:1119–1129
Elsharaky AS, Newairy AA, Badreldeen MM, Eweda SM, Sheweita SA (2007) Protective role of selenium against renal toxicity induced by cadmium in rats. Toxicology 235:185–193
Lazarus M, Orct T, Jurasoviæ J, Blanuša M (2009) The effect of dietary selenium supplementation on cadmium absorption and retention in suckling rats. Biometals 22:973–983
Gonzalez FJ (2005) Role of cytochromes P450 in chemical toxicity and oxidative stress: studies with CYP2E1. Mutat Res 569:101–110
Acknowledgments
We also thank the members of the veterinary internal medicine laboratory at the College of Veterinary Medicine, Northeast Agricultural University for their help in collecting the samples. The authors also thank Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine.
Funding
This study was supported by the National Natural Science Foundation of China (31472161).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
The Institutional Animal Care and Use Committee of Northeast Agricultural University approved all procedures used in this experiment.
Additional information
All of the authors have read the manuscript and agreed to submit it in its current form for consideration for publication in the Biological Trace Element Research
Rights and permissions
About this article
Cite this article
Cong, Y., Chi, Q., Teng, X. et al. The Protection of Selenium Against Cadmium-Induced Mitochondrial Damage via the Cytochrome P450 in the Livers of Chicken. Biol Trace Elem Res 190, 484–492 (2019). https://doi.org/10.1007/s12011-018-1557-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-018-1557-x