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Producing Acetic Acid of Acetobacter pasteurianus by Fermentation Characteristics and Metabolic Flux Analysis

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Abstract

The acetic acid bacterium Acetobacter pasteurianus plays an important role in acetic acid fermentation, which involves oxidation of ethanol to acetic acid through the ethanol respiratory chain under specific conditions. In order to obtain more suitable bacteria for the acetic acid industry, A. pasteurianus JST-S screened in this laboratory was compared with A. pasteurianus CICC 20001, a current industrial strain in China, to determine optimal fermentation parameters under different environmental stresses. The maximum total acid content of A. pasteurianus JST-S was 57.14 ± 1.09 g/L, whereas that of A. pasteurianus CICC 20001 reached 48.24 ± 1.15 g/L in a 15-L stir stank. Metabolic flux analysis was also performed to compare the reaction byproducts. Our findings revealed the potential value of the strain in improvement of industrial vinegar fermentation.

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Funding

This work was supported by National Natural Science Foundation of China (31601465), Project of Hefei University of Technology (JZ2017YYPY0247/JZ2016YYPY0041), and Anhui Science and Technology Project (15CZZ03100).

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Authors and Affiliations

  1. School of Food Science and Engineering, Hefei University of Technology, No.193 Tunxi Road, Hefei City, 230009, Anhui Province, People’s Republic of China

    Xuefeng Wu, Hongli Yao, Qing Liu, Zhi Zheng, Lili Cao, Dongdong Mu, Hualin Wang, Shaotong Jiang & Xingjiang Li

  2. Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei, 230009, Anhui Province, People’s Republic of China

    Xuefeng Wu, Zhi Zheng, Lili Cao, Hualin Wang, Shaotong Jiang & Xingjiang Li

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  1. Xuefeng Wu
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  2. Hongli Yao
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  3. Qing Liu
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Correspondence to Xingjiang Li.

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Wu, X., Yao, H., Liu, Q. et al. Producing Acetic Acid of Acetobacter pasteurianus by Fermentation Characteristics and Metabolic Flux Analysis. Appl Biochem Biotechnol 186, 217–232 (2018). https://doi.org/10.1007/s12010-018-2732-4

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