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Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines

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Abstract

The rapid growth of global biopharmaceutical market in the recent years has been a good indication of its significance in biotechnology industry. During a long period of time in recombinant protein production from 1980s, optimizations in both upstream and downstream processes were launched. In this regard, one of the most promising strategies is expression vector engineering technology based on incorporation of DNA opening elements found in the chromatin border regions of vectors as well as targeting gene integration. Along with these approaches, cell line engineering has revealed convenient outcomes in isolating high-producing clones. According to the fact that more than 50% of the approved therapeutic proteins is being manufactured in mammalian cell lines, in this review, we focus on several approaches and developments in vector and cell line engineering technologies in mammalian cell culture.

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Acknowledgements

We would like to thank Dr. Alireza Yousefzadeh for editing this manuscript. This research did not receive any specific grant from funding agencies in the public, commercial, or non-profit sectors.

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Authors and Affiliations

  1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Seyedeh Hoda Jazayeri

  2. Department of Genetics, Reproductive Biomedicine Research Center, ACECR, Royan Institute for Reproductive Biomedicine, P.O. Box: 14155-6343, Tehran, Iran

    Seyedeh Hoda Jazayeri, Amir Amiri-Yekta, Salahadin Bahrami, Hamid Gourabi & Mohammad Hossein Sanati

  3. Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

    Mohammad Hossein Sanati

  4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, P.O. Box: 1411413137, Tehran, Iran

    Mohammad Reza Khorramizadeh

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  1. Seyedeh Hoda Jazayeri
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  2. Amir Amiri-Yekta
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Correspondence to Mohammad Hossein Sanati or Mohammad Reza Khorramizadeh.

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Jazayeri, S.H., Amiri-Yekta, A., Bahrami, S. et al. Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines. Appl Biochem Biotechnol 185, 986–1003 (2018). https://doi.org/10.1007/s12010-017-2689-8

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