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Purification, Characterization of Amylase from Indigenously Isolated Aureobasidium pullulans Cau 19 and Its Bioconjugates with Gold Nanoparticles

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Abstract

The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal ions on the purified enzyme was investigated; 1 mM calcium (Ca) and cobalt (Co) enhanced enzyme activity by twofold; copper (Cu) had no effect on the activity of the enzyme. Mercury (Hg) 1 mM caused 90% inactivation whereas iron (Fe) and manganese (Mn) caused 10 to 16% inhibition. Amylase from A. pullulans Cau 19 was bioconjugated to gold nanoparticles synthesized using the biomass of A. pullulans Cau 19. Fourier transform infrared spectroscopy confirmed the conjugation of the enzyme to the gold nanoparticles. Though, only 20% of the added enzyme was adsorbed/conjugated on gold nanoparticles, 80% of the adsorbed activity could be estimated in the assay. The conjugated enzyme exhibited better tolerance to a broad pH range of 3.0–9.0 and higher temperatures compared with native enzyme.

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Acknowledgments

The authors are thankful to the BCUD, Savitribai Phule Pune University, Pune 411007, for providing funds. YM is thankful to the Department of Microbiology, Tuljaram Chaturchand College, for providing laboratory facilities.

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  1. Department of Microbiology, Tuljaram Chaturchand College, Baramati, Pune-413102, India

    Y.R. Mulay

  2. Department of Microbiology, Savitribai Phule Pune University, Pune, 411007, India

    Y.R. Mulay & R.L. Deopurkar

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Correspondence to R.L. Deopurkar.

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Mulay, Y., Deopurkar, R. Purification, Characterization of Amylase from Indigenously Isolated Aureobasidium pullulans Cau 19 and Its Bioconjugates with Gold Nanoparticles. Appl Biochem Biotechnol 184, 644–658 (2018). https://doi.org/10.1007/s12010-017-2575-4

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