Abstract
Glucose isomerase is the important enzyme for the production of high fructose corn syrup (HFCS). One-step production of HFCS containing more than 55% fructose (HFCS-55) is receiving much attention for its industrial applications. In this work, the Escherichia coli harboring glucose isomerase mutant TEGI-W139F/V186T was immobilized for efficient production of HFCS-55. The immobilization conditions were optimized, and the maximum enzyme activity recovery of 92% was obtained. The immobilized glucose isomerase showed higher pH, temperature, and operational stabilities with a K m value of 272 mM and maximum reaction rate of 23.8 mM min−1. The fructose concentration still retained above 55% after the immobilized glucose isomerase was reused for 10 cycles, and more than 85% of its initial activity was reserved even after 15 recycles of usage at temperature of 90 °C. The results highlighted the immobilized glucose isomerase as a potential biocatalyst for HFCS-55 production.
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Acknowledgements
The authors gratefully acknowledge the National Natural Science Foundation of China (No. 21602199; No. 31401527) and the Research Program of Science and Technology Department of Zhejiang Province (No. 2015C32052).
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Jin, LQ., Xu, Q., Liu, ZQ. et al. Immobilization of Recombinant Glucose Isomerase for Efficient Production of High Fructose Corn Syrup. Appl Biochem Biotechnol 183, 293–306 (2017). https://doi.org/10.1007/s12010-017-2445-0
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DOI: https://doi.org/10.1007/s12010-017-2445-0