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Microbial Community Structure of Activated Sludge for Biosolubilization of Two Different Rock Phosphates

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Abstract

A microbial consortium was directly taken from activated sludge and was used to solubilize rock phosphates (RPs) in a lab-scale bioreactor in this study. Results showed that the microbial consortium could efficiently release soluble phosphorus (P) from the RPs, and during 30-day incubation, it grew well in the bioreactor and reduced the pH of the solutions. The biosolubilization process was also illustrated by the observation of scanning electron microscopy combined with an energy dispersive X-ray spectroscopy (SEM–EDX), which showed an obvious corrosion on the ore surfaces, and most elements were removed from the ore samples. The analysis of microbial community structure by Illumina 16S ribosomal RNA (rRNA) gene and 18S rRNA gene MiSeq sequencing reflected different microbial diversity and richness in the solutions added with different ore samples. A lower richness and diversity of bacteria but a higher richness and diversity of fungi occurred in the solution added with ore sample 1 compared to that of in the solution added with ore sample 2. Alphaproteobacteria and Saccharomycetes were the dominating bacterial and fungal group, respectively, both in the solutions added with ore samples 1 and 2 at the class level. However, their abundances in the solution added with ore sample 1 were obviously lower than that in the solution added with ore sample 2. This study provides new insights into our understanding of the microbial community structure in the biosolubilization of RPs by a microbial consortium directly taken from activated sludge.

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Acknowledgements

This research work was kindly supported by the National Natural Science Foundation of China (51674178) and the Program for Excellent Young Scientific and Technological Innovation Team of Hubei Provincial Department of Education, China (T201506). The authors also thank Prof. Tong Yu, Prof. Zhenghe Xu, and Dr. Lei Zhu of the University of Alberta for their kindly support.

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  1. Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan, Hubei, 430073, China

    Chunqiao Xiao, Xiaoyan Wu, Tingting Liu, Guang Xu & Ruan Chi

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  1. Chunqiao Xiao
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  2. Xiaoyan Wu
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  4. Guang Xu
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Correspondence to Ruan Chi.

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Xiao, C., Wu, X., Liu, T. et al. Microbial Community Structure of Activated Sludge for Biosolubilization of Two Different Rock Phosphates. Appl Biochem Biotechnol 182, 742–754 (2017). https://doi.org/10.1007/s12010-016-2358-3

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  • DOI: https://doi.org/10.1007/s12010-016-2358-3

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