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Flanking Sequence Determination and Event Specific Detection of Transgenic Wheat B72-8-11b Strain

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Abstract

Exogenous fragment sequence and flanking sequence between exogenous fragment and recombinant chromosome of transgenic wheat B72-8-11b were successfully acquired through PCR amplification with cross-matched primers from exogenous genes. Newly acquired exogenous fragment covered the full-length sequence of transformed genes such as transformed plasmid and corresponding functional genes including marker uidA, promoter ubiquitin, lacZ, 1Dx5, and part of sequence of the wheat genome. A specific PCR detection method for transgenic wheat B72-8-11b strain was established on the basis of primers designed according to flanking sequence. The designed primers revealed specific amplification of 132 bp product of transgenic wheat B72-8-11b strain. This method is characteristics of high specificity, high reproducibility, rapid identification, and excellent accuracy for the identification of transgenic wheat B72-8-11b strain.

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Acknowledgments

This study was financially supported by important national science and technology-specific projects of China (2008ZX08012-001 and 2011ZX08012-001).

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Authors and Affiliations

  1. Liaoning Normal University, No.1 Liushu South Street, Gan Jing Zi District, Dalian, 116029, China

    Piqiao Zhang & Hesheng Hou

  2. Liaoning Entry–Exit Inspection and Quarantine Bureau, No.60 Changjiang East Road, Dalian, 116001, China

    Piqiao Zhang, Junyi Xu, Qiuyue Zheng & Jijuan Cao

  3. Heilongjiang Entry–Exit Inspection and Quarantine Bureau, Haerbin, 150001, China

    Fengxia Luan

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  1. Piqiao Zhang
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  2. Junyi Xu
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  3. Qiuyue Zheng
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  4. Fengxia Luan
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  5. Jijuan Cao
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  6. Hesheng Hou
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Correspondence to Jijuan Cao or Hesheng Hou.

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Zhang, P., Xu, J., Zheng, Q. et al. Flanking Sequence Determination and Event Specific Detection of Transgenic Wheat B72-8-11b Strain. Appl Biochem Biotechnol 169, 1523–1530 (2013). https://doi.org/10.1007/s12010-012-9989-9

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  • DOI: https://doi.org/10.1007/s12010-012-9989-9

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