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Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca

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Abstract

Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water solubility with 1,3-PD, not only contests the carbon flow and NADH with 1,3-PD but also serves as an obstacle for obtaining high purity 1,3-PD in downstream processes. In this study, a 2,3-BD pathway-deficient mutant of Klebsiella oxytoca ZG36 was constructed by knocking out the budA gene of the wild-type strain M5al. The results of fed-batch fermentation by ZG36 indicated that the glycerol flux and the distribution of metabolites were altered in the K. oxytoca when the 2,3-BD pathway was blocked. No 2,3-BD was produced, and the activity of α-acetolactate decarboxylase (α-ALDC) can not be detected in the fermentation processes. The indexes of the 1,3-PD titer, the conversion from glycerol to 1,3-PD, and the productivity per cell dry weight (CDW) increased by 42%, 62%, and 46%, respectively, compared with the M5al, and the yield of the byproducts also increased obviously. The assay of the enzyme activities in the oxidative branch and the reductive branch of the glycerol metabolism, as well as the intracellular redox state, exposited the results logically.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30900029).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People’s Republic of China

    Gang Zhang, Xu Wang, Qingjuan Guo, Ying Li & Jilun Li

  2. Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People’s Republic of China

    Gang Zhang, Xu Wang, Qingjuan Guo, Ying Li & Jilun Li

  3. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32 Xiqi Road, Tianjin Airport Economic Park, Tianjin, 300308, People’s Republic of China

    Guang Yang

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  1. Gang Zhang
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Correspondence to Guang Yang or Jilun Li.

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Zhang, G., Yang, G., Wang, X. et al. Influence of Blocking of 2,3-Butanediol Pathway on Glycerol Metabolism for 1,3-Propanediol Production by Klebsiella oxytoca . Appl Biochem Biotechnol 168, 116–128 (2012). https://doi.org/10.1007/s12010-011-9363-3

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  • DOI: https://doi.org/10.1007/s12010-011-9363-3

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