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Avantages et inconvénients du dosage de la 25-hydroxyvitamine D par chromatographie liquide couplée à la spectrométrie de masse en tandem (CL-SM/SM)

Strengths and limitations of the measurement of 25-hydroxyvitamin D by liquid chromatography — tandem mass spectrometry (LC-MS/MS)

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Bio tribune magazine

Résumé

Avec l’explosion ces dernières années de la demande, a des fins cliniques ou de recherche, de dosage de la 25-hydroxyvitamine D, le choix de la meilleure méthode pour un laboratoire de biologie médicale peut être particulièrement difficile avec le nombre important de méthodes actuellement disponibles. Les immunodosages offrent le meilleur compromis de simplicité, débit d’échantillons, coût et qualité de résultat, mais souffrent de limitations qui font de la CL-SM/SM la technique de référence, bien que plus coûteuse, pour sa flexibilité, sa sensibilité et sa spécificité.

Abstract

Given the dramatic increase, over the last few years, of both routine clinical and research use of the measurement of 25-hydroxyvitamin D, the choice of which is the best method, for an individual clinical laboratory, might be challenging especially given the range of methodologies actually available. Immunoassays offer the best compromise of convenience, high throughput, cost and good result, but also present notable limits that make the LC-MS/MS the gold standard due to its flexibility, sensitivity and specificity, although more expensive.

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Authors and Affiliations

  1. Service de Biochimie, Hôpital Saint-Louis, 1, Avenue Claude Vellefaux, 75010, Paris, France

    J. Fonsart

  2. Technicienne de laboratoire, Service de Biochimie, Hôpital Saint-Louis, 1, Avenue Claude Vellefaux, 75010, Paris, France

    I. Souletie

  3. Praticien Hospitalier, Service de Biochimie, Hôpital Saint-Louis, 1, Avenue Claude Vellefaux, 75010, Paris, France

    B. Gourmel

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Correspondence to J. Fonsart.

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Fonsart, J., Souletie, I. & Gourmel, B. Avantages et inconvénients du dosage de la 25-hydroxyvitamine D par chromatographie liquide couplée à la spectrométrie de masse en tandem (CL-SM/SM). Bio trib. mag. 39, 19–28 (2011). https://doi.org/10.1007/s11834-011-0054-8

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  • DOI: https://doi.org/10.1007/s11834-011-0054-8

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