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Glutathione S-Transferase (GST) Identified from Giant Kelp Macrocystis pyrifera Increases the Copper Tolerance of Synechococcus elongatus PCC 7942

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Abstract

The glutathione S-transferases gene family plays an important regulatory role in growth and development, and responses to environmental change. In this study, six complete GST genes (MpGST1, MpGST2, MpGST3, MpGST4, MpGST5, and MpGST6) were cloned from the gametophytes of brown alga Macrocystis pyrifera. Subsequent bioinformatics analysis showed that these six genes encoded proteins with 202, 216, 288, 201, 205, and 201 aa, respectively. Moreover, MpGST3 differs from the other GST genes. Phylogenetic analysis suggested that MpGST3 belongs to the Ure2p type GST. Domain analysis suggested that the other GSTs from M. pyrifera belong to the soluble GST family and form an independent branch with the GSTs found in the other macroalgae, suggesting that a new GST type was formed during macroalgal evolution. GST genes were upregulated in M. pyrifera when 2.5 mg L−1 Cu ions were added to the medium. Six GST genes were integrated into the genome of Synechococcus elongatus PCC 7942, and their functions were verified by measuring light absorbance, photosynthetic pigment content, and photosynthetic parameters of the transformed strains under 0.3 mg L−1 Cu ion stress. The results showed much higher levels of various parameters in the transformed strains than in the wild strain. The transformed strains (with the MpGST genes) showed significantly enhanced resistance to Cu ion stress, while the wild strain almost died. The results of this study lay a theoretical foundation for further research on the Cu ion stress resistance function of GSTs in M. pyrifera.

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Data Availability Statement

The raw data generated in this study were deposited in Genbank with accession numbers OL362284, OL362285, OL362286, OL362287, OL362288, OL362289.

Abbreviations

GSH:

glutathione

GST:

glutathione S-transferase

ROS:

reactive oxygen species

PCR:

polymerase chain reaction

qPCR:

real-time quantitative polymerase chain reaction

OD750 :

optical density at 750 nm

Chla :

chlorophyll a

Car:

carotenoid

CDS:

coding protein sequence

PI:

isoelectric point

PSII:

photosystem II

CK:

control

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Acknowledgements

This study is supported by the National Key R&D Program of China (No. 2018YFD0900305), the National Natural Science Foundation of China (No. 31770393), the Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program (No. 2019JZZY020706), the Central Public-Interest Scientific Institute Basal Research Fund, CAFS (Nos. 2020TD 19 and 2020TD27), the China Agriculture Research System (CARS-50), the Taishan Scholars Funding of Shandong Province, and the Taishan Scholars Funding and Talent Projects of Distinguished Scientific Scholars in Agriculture and Young Taishan Scholars Program to DONG Xu.

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  1. College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Zipeng Gu, Yudong Ren, Chengwei Liang & Yilin Geng

  2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Xiaowen Zhang, Dong Xu & Naihao Ye

  3. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Xiaowen Zhang, Dong Xu & Naihao Ye

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  1. Zipeng Gu
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Correspondence to Chengwei Liang or Naihao Ye.

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Gu, Z., Ren, Y., Liang, C. et al. Glutathione S-Transferase (GST) Identified from Giant Kelp Macrocystis pyrifera Increases the Copper Tolerance of Synechococcus elongatus PCC 7942. J. Ocean Univ. China 22, 777–789 (2023). https://doi.org/10.1007/s11802-023-5372-4

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