Abstract
This study was carried out in a tissue culture medium using two salt stress-tolerant varieties (Oryza sativa L. cv. Dhan47 and Kızıltan mutant8) and two stress resistance varieties (Oryza sativa L. cv. Maraş Yellow Rice and Kızıltan) at 50 mM, 100 mM and 200 mM NaCl concentrations in two different periods (fifteen and thirty days). In this study, we analysed root and stem length, antioxidant enzyme activities and gene expressions. Among the varieties, the highest size was observed in the maraş variety, and the least length was observed in the dhan47 rice varieties in the 30-day stem length results. While there was no adverse effect on root lengths as much as 100 mm, it was observed that it had a negative impact on a 200 mM medium. In the 30-day results, it was observed that the SOD activity in the leaves of the Kızıltan variety decreased compared to the control, while it was observed that the activity increased up to 100 mM salt concentration in the roots. It was determined that CAT activity increased in leaves and roots at all concentrations compared to the control at increasing salt concentrations over a 15-day period. At the same time, it was observed that CAT activity was the highest at 100 mM salt concentration on the leaves of dhan 47 plant. In the 30-day results, it was observed that FDH activity increased in leaves and roots in all rice varieties compared to the control. In gene expression analyses, the highest (7 times) gene expression in roots was observed on Kızıltan100 mM salt concentration. The highest relative expression (14 times) in leaves was on the Kiziltan mutant leaf at 200 mM salt concentration. The results showed different responses in physiological effects, antioxidant activities and gene expression analyses as salt concentrations of all rice varieties. With the results obtained in this study, it is expected that different rice varieties that are sensitive and resistant to salt stress will contribute to the usability of agricultural lands where salinity is gradually increasing.
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Data availability
The authors confirm that data supporting the findings of this study are available in the article and Supplementary material. All data are included in Yunus Emre Arvas doctoral (Ph.D.) thesis. Raw data supporting the findings of this study are available from the corresponding author upon reasonable request.
Abbreviations
- ROS:
-
Reactive oxygen species
- GR:
-
Glutathione reductase
- POX:
-
Peroxidase
- SOD:
-
Superoxide dismutase
- GPX:
-
Guaiacol peroxidase
- CAT:
-
Catalase
- APX:
-
Ascorbate peroxidase
- FDH:
-
Formate dehydrogenase
- NAD+ :
-
Nikotinamid adenin dinücleotide
- MSR:
-
Methionine sulfoxide reductase
- EDTA:
-
Ethylene diamine tetraacetic acid
- Ms:
-
Murashige and Skoog medium
- PBS:
-
Phosphate-buffered saline
- BSA:
-
Bovine serum albumin
- mM:
-
Milimolar
- NBT:
-
Nitro blue tetrazole
- PMS:
-
Phenazine methosulfate
- ANOVA:
-
Analysis of variance
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Acknowledgements
This report is a part of the Ph.D. Thesis of Yunus Emre Arvas, Ph.D. Candidate in Molecular Biology and Genetics Department, Yildiz Technical University. This study was supported by the Research Foundation of Yildiz Technical University, Project No: FBA-2020-3901
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All authors contributed to the study’s conception and design. Material preparation, data collection and analysis were performed by Yunus Emre Arvas, İsmail Kocacaliskan, Emel Ordu and Semiha Erişen. Yunus Emre Arvas and Emel Ordu wrote the first draft of the manuscript, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Arvas, Y.E., Kocacaliskan, İ., Erisen, S. et al. Antioxidant and molecular response of mutant and native rice (Oryza sativa L.) varieties grown under salt stress. Biologia 78, 1199–1210 (2023). https://doi.org/10.1007/s11756-023-01342-5
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DOI: https://doi.org/10.1007/s11756-023-01342-5