Abstract
Demand for probiotics has increased significantly in recent years due to the growing consumer awareness of their benefits and advantages. In this study, we investigated the probiotic potential use of three antifungal lactic acid bacteria (LAB) strains namely Lactiplantibacillus pentosus 22B, Lactiplantibacillus plantarum 21B and Enterococcus faecium LC2V5 isolated from wheat and camel milk. A series of in vitro probiotic tests were performed to evaluate their functional and safety properties. Results indicated that consumption of these three strains does not present any health risk, as they showed no undesirable virulence factors (hemolysins and DNAse) or transmissible antibiotic resistance. All strains exhibited high tolerance to simulated oro-gastro-intestinal tract conditions since the number of viable cells was 109 CFU/mL after exposure to these digestive stress environments. Evaluation of cell surface characteristics revealed that the three strains showed high degrees of auto-aggregation, hydrophobicity and co-aggregation with pathogens. Moreover, the strains demonstrated strong antagonistic activity against all tested pathogenic bacteria (Salmonella typhimurium, Listeria monocytogenes, Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii and Pseudomonas aeruginosa). Furthermore, they were able to produce exopolysaccharides and ferment lactose. The studied strains showed also antioxidant activity by scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. In conclusion, the probiotic features demonstrated by the studied LAB strains suggest their suitability for potential use as probiotics.
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Abbreviations
- LAB:
-
Lactic acid bacteria
- CFU:
-
Colony forming unit
- DPPH:
-
1,1-diphenyl-2-picrylhydrazyl)
- FAO:
-
Food and Agriculture Organization of the United Nations
- WHO:
-
World Health Organization
- MRS agar:
-
De Man, Rogosa and Sharpe agar
- PBS:
-
Phosphate-Buffered Saline
- OD:
-
Optical density
- E 15:
-
15 μg Erythromycin
- TE 30:
-
30 μg Tetracycline
- AMP 10:
-
10 μg Ampicillin
- S 25:
-
25 μg Streptomycin
- FOS 50:
-
50 μg Fosfomycin
- IPM 10:
-
10 μg Imipenem
- KF 30:
-
30 μg Cephalothin
- K 30:
-
30 μg Kanamycin
- CN 10:
-
10 μg Gentamicin
- SXT 25:
-
25 μg Trimethoprim/Sulfamethoxazole
- SAM 20:
-
20 μg Ampicillin/Sulbactam
- EPS:
-
Exopolysaccharides
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This study was supported by “Centre National pour la Recherche Scientifique et Technique” under Grant Agreement No [PPR2/2016/07].
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The authors confirm contributions to the paper as follows: Study conception and design: Tarik Lakhlifi; Material preparation and analysis: Tarik Lakhlifi and Samia El oirdi; Writing - original draft preparation: Tarik Lakhlifi; Writing - review and editing: Abdelhaq Belhaj, Itto Maroui, Rachid Zouhair; Supervision: Abdelhaq Belhaj. All authors read and approved the final manuscript.
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Lakhlifi, T., El oirdi, S., Maroui, I. et al. Probiotic properties and safety aspect of three antifungal lactic acid bacteria strains isolated from wheat and camel milk. Biologia 78, 1129–1139 (2023). https://doi.org/10.1007/s11756-023-01319-4
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DOI: https://doi.org/10.1007/s11756-023-01319-4