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Sequential Feeding of Lipid Supplement Enriches Beef Adipose Tissues with 18:3n-3 Biohydrogenation Intermediates

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Lipids

Abstract

The present study was designed to determine if feeding steers extruded flaxseed and hay (25 and 75%; DM basis) together as a total mixed ration (TMR), or sequentially (non-TMR) would result in different enrichments of polyunsaturated fatty acids (PUFA) and their biohydrogenation intermediates (BHI) in beef adipose tissues [subcutaneous (SC) vs perirenal (PR) fat]. Forty-eight Angus cross steers (325 ± 16 kg) were stratified by weight to six pens, and pens were randomized to either TMR or non-TMR and fed ad libitum for an average of 242 days. The concentrations of α-linolenic acid increased by 18 mol% in both SC and PR in non-TMR steers compared to TMR steers (P < 0.01). trans 18:1 isomers were more concentrated in PR than SC (14.4 vs 9.5 mol%; P < 0.01) and increased by 10 mol% in both fat depots for non-TMR (P < 0.01). Other BHI including non-methylene-interrupted 18:2 (atypical dienes), conjugated linoleic acids and conjugated linolenic acids (CLnA) were affected by diet × tissue interactions (P < 0.01). The CLnA and CLA contents were higher in both fat depots when feeding the non-TMR, but the effect of diet was more pronounced in PR than in SC (P < 0.01). Atypical dienes were highest in PR from non-TMR and lowest in TMR fed steers (4.3 and 3.6 mol%) with SC contents being intermediate. The sequential feeding of lipid supplement can thus profoundly affect the enrichment of PUFA and their BHI in beef fat and their differentially enrichment is also fat depot dependant.

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Abbreviations

ALA:

α-Linolenic acid

ANOVA:

Analysis of variance

AD:

Atypical dienes

BCFA:

Branched-chain fatty acids

BHI:

Biohydrogenation intermediates

c :

cis

CLA:

Conjugated linoleic acid

CLnA:

Conjugated linolenic acid

DM:

Dry matter

FA:

Fatty acids

FAME:

Fatty acid methyl esters

GC:

Gas chromatography

HDL:

High density lipoproteins

LDL:

Low density lipoproteins

LNA:

Linoleic acid

MUFA:

Monounsaturated fatty acids

n-3:

Omega-3

n-6:

Omega-6

PR:

Perirenal

PUFA:

Polyunsaturated fatty acids

RA:

Rumenic acid

SC:

Subcutaneous

SFA:

Saturated fatty acids

TMR:

Total mixed ration

t :

trans

VA:

Vaccenic acid

References

  1. McAfee AJ, McSorley EM, Cuskelly GJ, Moss BW, Wallace JMW, Bonham MP, Fearon AM (2010) Red meat consumption: an overview of the risks and benefits. Meat Sci 84:1–13

    Article  CAS  PubMed  Google Scholar 

  2. Wood JD, Enser M, Fisher AV, Nute GR, Richardson RI, Sheard PR (1999) Manipulating meat quality and composition. Proc Nutr Soc 58:363–370

    Article  CAS  PubMed  Google Scholar 

  3. Agriculture and Agrifood Canada (2015) Per capita consumption. Protein disappearance of poultry and other animal protein sources in Canada. http://www.agr.gc.ca/eng/industry-markets-and-trade/statistics-and-market-information. Accessed 15 Jan 2017

  4. de Souza RJ, Mente A, Maroleanu A, Cozma AI, Ha V, Kishibe T, Uleryk E, Budylowski P, Schünemann H, Beyene J, Anand SS (2015) Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studies. BMJ 351:h3978

    Article  PubMed  PubMed Central  Google Scholar 

  5. Siri-Tarino PW, Chiu S, Bergeron N, Krauss RM (2015) Saturated fats versus polyunsaturated fats versus carbohydrates for cardiovascular disease prevention and treatment. Annu Rev Nutr 35:517–543

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Howe PR, Meyer BJ, Record S, Baghurst K (2003) Contribution of red meat to very long chain omega-3 fatty acid (VLCOmega3) intake. Asia Pac J Clin Nutr 12:S27

    Google Scholar 

  7. Kim JH, Kim Y, Kim YJ, Park Y (2016) Conjugated linoleic acid: potential health benefits as a functional food ingredient. Annu Rev Food Sci Technol 7:221–244

    Article  CAS  PubMed  Google Scholar 

  8. Field CJ, Blewett HH, Proctor S, Vine D (2009) Human health benefits of vaccenic acid. Appl Physiol Nutr Metab 34:979–991

    Article  CAS  PubMed  Google Scholar 

  9. Jenkins T, Wallace R, Moate P, Mosley E (2008) Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J Anim Sci 86:397–412

    Article  CAS  PubMed  Google Scholar 

  10. Dugan M, Aldai N, Aalhus J, Rolland D, Kramer J (2011) Review: trans-forming beef to provide healthier fatty acid profiles. Can J Anim Sci 91:545–556

    Article  CAS  Google Scholar 

  11. Vahmani P, Mapiye C, Prieto N, Rolland DC, McAllister TA, Aalhus JL, Dugan ME (2015) The scope for manipulating the polyunsaturated fatty acid content of beef: a review. J Anim Sci Biotechnol 6:29

    Article  PubMed  PubMed Central  Google Scholar 

  12. Mapiye C, Aalhus JL, Turner TD, Rolland DC, Basarab JA, Baron VS, McAllister TA, Block HC, Uttaro B, Lopez-Campos O, Proctor SD, Dugan ME (2013) Effects of feeding flaxseed or sunflower-seed in high-forage diets on beef production, quality and fatty acid composition. Meat Sci 95:98–109

    Article  CAS  PubMed  Google Scholar 

  13. Mapiye C, Turner T, Rolland D, Basarab J, Baron V, McAllister T, Block H, Uttaro B, Aalhus J, Dugan M (2013) Adipose tissue and muscle fatty acid profiles of steers fed red clover silage with and without flaxseed. Livest Sci 151:11–20

    Article  Google Scholar 

  14. Mapiye C, Aalhus JL, Turner TD, Rolland DC, Basarab JA, Baron VS, McAllister TA, Block HC, Proctor SD, Dugan ME (2014) Types of oilseed and adipose tissue influence the composition and relationships of polyunsaturated fatty acid biohydrogenation products in steers fed a grass hay diet. Lipids 49:275–286

    Article  CAS  PubMed  Google Scholar 

  15. Turner TD, Aalhus JL, Mapiye C, Rolland DC, Larsen IL, Basarab JA, Baron VS, McAllister TA, Block HC, Uttaro B, Dugan ME (2015) Effects of diets supplemented with sunflower or flax seeds on quality and fatty acid profile of hamburgers made with perirenal or subcutaneous fat. Meat Sci 99:123–131

    Article  CAS  PubMed  Google Scholar 

  16. Vahmani P, Rolland DC, McAllister TA, Block HC, Proctor SD, Guan LL, Prieto N, Aalhus JL, Dugan MER (2017) Effects of feeding steers extruded flaxseed mixed with hay or before hay on animal performance, carcass quality, and meat and hamburger fatty acid composition. Meat Sci In Press. doi:10.1016/j.meatsci.2017.04.008

    Google Scholar 

  17. Canadian Council on Animal Care guidelines on the care and use of farm animals in research, teaching and testing Canadian Council on Animal Care (CCAC), Ottawa, ON. http://www.ccac.ca/Documents/Standards/Guidelines/Farm_Animals.pdf. Accessed 15 Jan 2017

  18. Aldai N, Dugan MER, Rolland DC, Kramer JKG (2009) Survey of the fatty acid composition of Canadian beef: backfat and longissimus lumborum muscle. Can J Anim Sci 89:315–329

    Article  CAS  Google Scholar 

  19. Kramer JKG, Hernandez M, Cruz-Hernandez C, Kraft J, Dugan MER (2008) Combining results of two GC separations partly achieves determination of all cis and trans 16:1, 18:1, 18:2 and 18:3 except CLA isomers of milk fat as demonstrated using ag-ion SPE fractionation. Lipids 43:259–273

    Article  CAS  PubMed  Google Scholar 

  20. Turner T, Rolland D, Aldai Elkoro-Iribe N, Dugan M (2011) Short communication: rapid separation of cis9, trans11-and trans7, cis9-18: 2 (CLA) isomers from ruminant tissue using a 30 m SLB-IL111 ionic column. Can J Anim Sci 91:711–713

    Article  CAS  Google Scholar 

  21. Cruz-Hernandez C, Deng Z, Zhou J, Hill AR, Yurawecz MP, Delmonte P, Mossoba MM, Dugan MER, Kramer JKG (2004) Methods for analysis of conjugated linoleic acids and trans-18:1 isomers in dairy fats by using a combination of gas chromatography, silver-ion thin-layer chromatography/gas chromatography, and silver-ion liquid chromatography. J AOAC Int 87:545–562

    CAS  PubMed  Google Scholar 

  22. Gómez-Cortés P, Bach A, Luna P, Juárez M, de la Fuente MA (2009) Effects of extruded linseed supplementation on n-3 fatty acids and conjugated linoleic acid in milk and cheese from ewes. J Dairy Sci 92:4122–4134

    Article  PubMed  Google Scholar 

  23. Vahmani P, Rolland DC, Gzyl KE, Dugan MER (2016) Non-conjugated cis/trans 18:2 in beef fat are mainly Δ-9 desaturation products of trans-18:1 isomers. Lipids 51:1427–1433

    Article  CAS  PubMed  Google Scholar 

  24. SAS (2009) SAS user’s guide: Statistics. SAS for windows. Release 9.2. SAS Institute Inc, Cary

    Google Scholar 

  25. Juarez M, Dugan ME, Aalhus JL, Aldai N, Basarab JA, Baron VS, McAllister TA (2011) Effects of vitamin E and flaxseed on rumen-derived fatty acid intermediates in beef intramuscular fat. Meat Sci 88:434–440

    Article  CAS  PubMed  Google Scholar 

  26. LaBrune H, Reinhardt C, Dikeman M, Drouillard J (2008) Effects of grain processing and dietary lipid source on performance, carcass characteristics, plasma fatty acids, and sensory properties of steaks from finishing cattle. J Anim Sci 86:167–172

    Article  CAS  PubMed  Google Scholar 

  27. Nassu RT, Dugan ME, He ML, McAllister TA, Aalhus JL, Aldai N, Kramer JK (2011) The effects of feeding flaxseed to beef cows given forage based diets on fatty acids of longissimus thoracis muscle and backfat. Meat Sci 89:469–477

    Article  CAS  PubMed  Google Scholar 

  28. Martínez Marín AL, Gómez-Cortés P, Gómez Castro AG, Juárez M, Pérez Alba LM, Pérez Hernández M, de la Fuente MA (2011) Animal performance and milk fatty acid profile of dairy goats fed diets with different unsaturated plant oils. J Dairy Sci 94:5359–5368

    Article  PubMed  Google Scholar 

  29. Hennessy AA, Ross RP, Devery R, Stanton C (2011) The health promoting properties of the conjugated isomers of α-linolenic acid. Lipids 46:105–119

    Article  CAS  PubMed  Google Scholar 

  30. Palmquist DL, Lock AL, Shingfield KJ, Bauman DE (2005) Biosynthesis of conjugated linoleic acid in ruminants and humans. Adv Food Nutr Res 50:179–217

    Article  CAS  PubMed  Google Scholar 

  31. Hino S, and Fukuda T (2006) Biohydrogenation of linoleic and linolenic acids, and production of their conjugated isomers by Butyrivibrio fibrisolvens. 4th Euro Fed Lipid Congress Madrid, Spain, p 551

  32. Rioux V, Pédrono F, Blanchard H, Duby C, Boulier-Monthéan N, Bernard L, Beauchamp E, Catheline D, Legrand P (2013) Trans-Vaccenate is Δ13-desaturated by FADS3 in rodents. J Lipid Res 54:3438–3452

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Garcia C, Duby C, Catheline D, Toral PG, Bernard L, Legrand P, Rioux V (2017) Synthesis of the suspected trans-11, cis-13 conjugated linoleic acid isomer in ruminant mammary tissue by FADS3-catalyzed delta13-desaturation of vaccenic acid. J Dairy Sci 100:783–796

    Article  CAS  PubMed  Google Scholar 

  34. Noci F, French P, Monahan FJ, Moloney AP (2007) The fatty acid composition of muscle fat and subcutaneous adipose tissue of grazing heifers supplemented with plant oil-enriched concentrates. J Anim Sci 85:1062–1073

    Article  CAS  PubMed  Google Scholar 

  35. Degen C, Ecker J, Piegholdt S, Liebisch G, Schmitz G, Jahreis G (2011) Metabolic and growth inhibitory effects of conjugated fatty acids in the cell line HT-29 with special regard to the conversion of t11, t13-CLA. BBA Mol Cell Biol Lipids 1811:1070–1080

    Article  CAS  Google Scholar 

  36. Schneider A-C, Beguin P, Bourez S, Perfield JW II, Mignolet E, Debier C, Schneider Y-J, Larondelle Y (2012) Conversion of t11t13 cla into c9t11 cla in Caco-2 cells and inhibition by sterculic oil. PLoS One 7:e32824

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Jiang T, Mueller CJ, Busboom JR, Nelson ML, O’Fallon J, Tschida G (2013) Fatty acid composition of adipose tissue and muscle from Jersey steers was affected by finishing diet and tissue location. Meat Sci 93:153–161

    Article  CAS  PubMed  Google Scholar 

  38. Lee JH, Yamamoto I, Jeong JS, Nade T, Arai T, Kimura N (2011) Relationship between adipose maturity and fatty acid composition in various adipose tissues of Japanese Black, Holstein and Crossbred (F1) steers. Anim Sci J 82:689–697

    Article  CAS  PubMed  Google Scholar 

  39. Siurana A, Calsamiglia S (2016) A metaanalysis of feeding strategies to increase the content of conjugated linoleic acid (CLA) in dairy cattle milk and the impact on daily human consumption. Anim Feed Sci Technol 217:13–26

    Article  CAS  Google Scholar 

  40. Kennedy A, Martinez K, Schmidt S, Mandrup S, LaPoint K, McIntosh M (2010) Antiobesity mechanisms of action of conjugated linoleic acid. J Nutr Biochem 21:171–179

    Article  CAS  PubMed  Google Scholar 

  41. Turpeinen AM, Mutanen M, Aro A, Salminen I, Basu S, Palmquist DL, Griinari JM (2002) Bioconversion of vaccenic acid to conjugated linoleic acid in humans. Am J Clin Nutr 76:504–510

    CAS  PubMed  Google Scholar 

  42. Sofi F, Buccioni A, Cesari F, Gori AM, Minieri S, Mannini L, Casini A, Gensini GF, Abbate R, Antongiovanni M (2010) Effects of a dairy product (pecorino cheese) naturally rich in cis-9, trans-11 conjugated linoleic acid on lipid, inflammatory and haemorheological variables: a dietary intervention study. Nutr Metab Cardiovasc Dis 20:117–124

    Article  CAS  PubMed  Google Scholar 

  43. Mir PS, Okine EK, Goonewardene L, He ML, Mir Z (2003) Effects of synthetic conjugated linoleic acid (CLA) or bio-formed CLA as high CLA beef on rat growth and adipose tissue development. Can J Anim Sci 83:583–592

    Article  CAS  Google Scholar 

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Acknowledgments

This research was funded by the Alberta Meat and Livestock Agency (ALMA). The gift of linPRO-R™ from O&T Farms (Regina, SK, Canada) is gratefully acknowledged. Special thanks are extended to staff at the LaRDC Beef Unit for animal care, management and sample collection. The slaughter and processing of the cattle by the LaRDC abattoir staff is gratefully acknowledged, as are the contributions made by the meat grading and muscle biochemistry staff. Ms. I. L. Larsen is acknowledged for her valuable assistance in statistical analyses.

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Correspondence to M. E. R. Dugan.

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Vahmani, P., Aalhus, J.L., Rolland, D.C. et al. Sequential Feeding of Lipid Supplement Enriches Beef Adipose Tissues with 18:3n-3 Biohydrogenation Intermediates. Lipids 52, 641–649 (2017). https://doi.org/10.1007/s11745-017-4259-9

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