Abstract
The freshwater microalga Parietochloris incisa accumulates, under nitrogen starvation, large amounts of triacylglycerols containing approximately 60% of the ω6 very long-chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid. Based on sequence homology, we isolated three cDNA sequences from P. incisa, designated PiDesD12, PiDesD6, PiDesD5. The deduced amino acid sequences of the three genes contained three conserved histidine motifs; the front-end desaturases, PiDes6 and PiDes5, contained a fused N-terminal cytochrome b5 domain. By functional characterization in the yeast Saccharomyces cerevisiae, we confirmed that PiDesD6, PiDesD5 cDNA encode membrane bound desaturases with Δ6, and Δ5 activity, respectively. Both PiDes6 and PiDes5 can indiscriminately desaturate both ω6 and ω3 substrates. A phylogenetic analysis showed that the three genes were homologous to the corresponding desaturases from green microalgae and lower plants that were functionally characterized. Quantitative real-time PCR revealed the concerted expression pattern of all three genes in P. incisa cells subjected to nitrogen starvation, featuring maximum expression level on day 3 of starvation, corresponding to the sharpest increase in the share of arachidonic acid.
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Abbreviations
- ARA:
-
Arachidonic acid
- DGTS:
-
Diacylglyceroltrimethylhomoserine
- DHA:
-
Docosahexaenoic acid
- DGLA:
-
Dihomo-γ-linolenic acid
- EPA:
-
Eicosapentaenoic acid
- GLA:
-
γ-Linolenic acid
- LNA:
-
Linoleic acid
- PtdCho:
-
Phosphatidylcholine
- PtdEtn:
-
Phosphatidylethanolamine
- RTQPCR:
-
Real time quantitative polymerase chain reaction
- TFA:
-
Total fatty acids
- VLC-PUFA:
-
Very long-chain polyunsaturated fatty acids
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Acknowledgments
Authors would like to thank Mrs. Shoshana Didi-Cohen for the dedicated technical assistance.
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GenBank accession numbers for PiDes12, PiDes6 and PiDes5 and a partial sequence of actin gene of P. incisa are GU390531, GU390532, GU390533 and FJ548973.
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Iskandarov, U., Khozin-Goldberg, I. & Cohen, Z. Identification and Characterization of Δ12, Δ6, and Δ5 Desaturases from the Green Microalga Parietochloris incisa . Lipids 45, 519–530 (2010). https://doi.org/10.1007/s11745-010-3421-4
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DOI: https://doi.org/10.1007/s11745-010-3421-4