Skip to main content
SpringerLink
Log in

Regulation of the protein and gene expressions of ethylene biosynthesis enzymes under different temperature during peach fruit ripening

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Ethylene has profound effect on fruit development and ripening, and the role of ethylene biosynthesis enzymes involving 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS), ACC oxidase (ACO), and S-Adenosyl-l-methionine synthetase (SAMS) in peach fruit (cv. Xiahui-8) was characterized under 25 and 4 °C, respectively. All these enzymes in ethylene synthesis pathway were identified using 2-DE and real-time PCR. Both protein and gene expressions of ACO and SAMS were much higher at 25 °C than at 4 °C. Among five members of ACS family, PpaACS4 may belong to system II ethylene biosynthesis, while PpaACS3 involved in system I during development stage, and low temperature can induce PpaACS1 expression. The ethylene release and low expressions of proteins and genes of most enzymes indicated that low temperature can effectively postpone ripening stage by reducing ethylene evolution. High gene expression of PpaSAMS did not cause excessive expression of SAMS protein under low temperature, and over-expression of PpaACS1 at low temperature still did not induce increase of ethylene production. The mechanism underlying the phenomenon about how temperature affects ethylene release was also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Amoros A, Serrano M, Riquelme F, Romojaro F (1989) Levels of Acc and physical and chemical-parameters in peach development. J Hortic Sci 64:673–677

    Article  CAS  Google Scholar 

  • Barry CS, Llop-Tous MI, Grierson D (2000) The regulation of 1-aminocyclopropane-1-carboxylic acid synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiol 123:979–986

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Borsani J, Budde CO, Porrini L, Lauxmann MA, Lombardo VA, Murray R, Andreo CS, Drincovich MF, Lara MV (2009) Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications. J Exp Bot 60:1823–1837

    Article  CAS  PubMed  Google Scholar 

  • Campbell OE, Padilla-Zakour OI (2013) Phenolic and carotenoid composition of canned peaches (Prunus persica) and apricots (Prunus armeniaca) as affected by variety and peeling. Food Res Int 54:448–455

    Article  CAS  Google Scholar 

  • Crisosto CH, Day KR, Crisosto GM, Garner D (2001) Quality attributes of white flesh peaches and nectarines grown under California conditions. J Amer Pomolog Soc 55:45–51

    Google Scholar 

  • El-Sharkawy I, Sherif S, Qubbaj T, Sullivan AJ, Jayasankar S (2016) Stimulated auxin levels enhance plum fruit ripening, but limit shelf-life characteristics. Postharvest Biol Tec 112:215–223

    Article  CAS  Google Scholar 

  • Ferrer A, Remon S, Negueruela AI, Oria R (2005) Changes during the ripening of the very late season Spanish peach cultivar Calanda—feasibility of using CIELAB coordinates as maturity indices. Sci Hortic Amsterdam 105:435–446

    Article  CAS  Google Scholar 

  • Good X, Kellogg JA, Wagoner W, Langhoff D, Matsumura W, Bestwick RK (1994) Reduced ethylene synthesis by transgenic tomatoes expressing S-adenosylmethionine hydrolase. Plant Mol Biol 26:781–790

    Article  CAS  PubMed  Google Scholar 

  • Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346:284–287

    Article  CAS  Google Scholar 

  • Huan C, Jiang L, An X, Kang R, Yu M, Ma R, Yu Z (2016a) Potential role of glutathione peroxidase gene family in peach fruit ripening under combined postharvest treatment with heat and 1-MCP. Postharvest Biol Tec 111:175–184

    Article  CAS  Google Scholar 

  • Huan C, Jiang L, An X, Yu M, Xu Y, Ma R, Yu Z (2016b) Potential role of reactive oxygen species and antioxidant genes in the regulation of peach fruit development and ripening. Plant Physiol Bioch 104:294–303

    Article  CAS  Google Scholar 

  • Jiang L, Zhang L, Shi Y, Lu ZX, Yu ZF (2014) Proteomic analysis of peach fruit during ripening upon post-harvest heat combined with 1-MCP treatment. J Proteom 98:31–43

    Article  CAS  Google Scholar 

  • Kang RY, Zhang L, Jiang L, Yu ML, Ma RJ, Yu ZF (2016) Effect of postharvest nitric oxide treatment on the proteome of peach fruit during ripening. Postharvest Biol Tec 112:277–289

    Article  CAS  Google Scholar 

  • Katz E, Lagunes PM, Riov J, Weiss D, Goldschmidt EE (2004) Molecular and physiological evidence suggests the existence of a system II-like pathway of ethylene production in non-climacteric Citrus fruit. Planta 219:243–252

    Article  CAS  PubMed  Google Scholar 

  • Kim SB, Yu JG, Lee GH, Park YD (2012) Characterization of Brassica rapa S-adenosyl-l-methionine synthetase gene including its roles in biosynthesis pathway. Hortic Environ Biote 53:57–65

    Article  CAS  Google Scholar 

  • Kumar A, Altabella T, Taylor MA, Tiburcio AF (1997) Recent advances in polyamine research. Trends Plant Sci 2:124–130

    Article  Google Scholar 

  • Lelievre JM, Latche A, Jones B, Bouzayen M, Pech JC (1997) Ethylene and fruit ripening. Physiol Plantarum 101:727–739

    Article  CAS  Google Scholar 

  • Mathooko FM, Tsunashima Y, Owino WZO, Kubo Y, Inaba A (2001) Regulation of genes encoding ethylene biosynthetic enzymes in peach (Prunus persica L.) fruit by carbon dioxide and 1-methylcyclopropene. Postharvest Biol Tec 21:265–281

    Article  CAS  Google Scholar 

  • Mbeguie-A-Mbeguie D, Chahine H, Gomez RM, Gouble B, Reich M, Audergon JM, Souty M, Albagnac G, Fils-Lycaon B (1999) Molecular cloning and expression of a cDNA encoding 1-aminocyclopropane-1-carboxylate (ACC) oxidase from apricot fruit (Prunus armeniaca). Physiol Plantarum 105:294–303

    Article  CAS  Google Scholar 

  • McMurchie EJ, McGlasson WB, Eaks IL (1972) Treatment of fruit with propylene gives information about the biogenesis of ethylene. Nature 237:235–236

    Article  CAS  PubMed  Google Scholar 

  • Meng C, Yang DY, Ma XC, Zhao WY, Liang XQ, Ma NN, Meng QW (2016) Suppression of tomato SlNAC1 transcription factor delays fruit ripening. J Plant Physiol 193:88–96

    Article  CAS  PubMed  Google Scholar 

  • Munoz-Robredo P, Rubio P, Infante R, Campos-Vargas R, Manriquez D, Gonzalez-Aguero M, Defilippi BG (2012) Ethylene biosynthesis in apricot: identification of a ripening-related 1-aminocyclopropane-1-carboxylic acid synthase (ACS) gene. Postharvest Biol Tec 63:85–90

    Article  CAS  Google Scholar 

  • Nilo R, Campos-Vargas R, Orellana A (2012) Assessment of Prunus persica fruit softening using a proteomics approach. J Proteom 75:1618–1638

    Article  Google Scholar 

  • Oeller PW, Wong LM, Taylor LP, Pike DA, Theologis A (1991) Reversible inhibition of tomato fruit senescence by antisense Rna. Science 254:437–439

    Article  CAS  PubMed  Google Scholar 

  • Oetiker JH, Yang SF (1995) The role of ethylene in fruit ripening. Acta Hortic 398:167–178

    Article  CAS  Google Scholar 

  • Prinsi B, Negri AS, Fedeli C, Morgutti S, Negrini N, Cocucci M, Espen L (2011) Peach fruit ripening: a proteomic comparative analysis of the mesocarp of two cultivars with different flesh firmness at two ripening stages. Phytochemistry 72:1251–1262

    Article  CAS  PubMed  Google Scholar 

  • Rugkong A, Rose JKC, Lee SJ, Giovannoni JJ, O’Neill MA, Watkins CB (2010) Cell wall metabolism in cold-stored tomato fruit. Postharvest Biol Tec 57:106–113

    Article  CAS  Google Scholar 

  • Shi Y, Jiang L, Zhang L, Kang R, Yu Z (2014) Dynamic changes in proteins during Apple (Malus x domestica) fruit ripening and storage. Hortic Res 1:14

    Article  PubMed  PubMed Central  Google Scholar 

  • Sis SA, Mostofi Y, Boojar MMA, Khalighi A (2012) Effect of nitric oxide on ethylene biosynthesis and antioxidant enzymes on Iranian peach (Prunus persica cv. Anjiri). J Food Agric Environ 10:125–129

    CAS  Google Scholar 

  • Slapeta J, Stejskal F, Keithly JS (2003) Characterization of S-adenosylmethionine synthetase in Cryptosporidium parvum (Apicomplexa). FEMS Microbiol Lett 225:271–277

    Article  CAS  PubMed  Google Scholar 

  • Tatsuki M, Haji T, Yamaguchi M (2006) The involvement of 1-aminocyclopropane-1-carboxylic acid synthase isogene, Pp-ACS1, in peach fruit softening. J Exp Bot 57:1281–1289

    Article  CAS  PubMed  Google Scholar 

  • Tong ZG, Gao ZH, Wang F, Zhou J, Zhang Z (2009) Selection of reliable reference genes for gene expression studies in peach using real-time PCR. BMC Mol Biol 10:71

    Article  PubMed  PubMed Central  Google Scholar 

  • Torrigiani P, Bressanin D, Ruiz KB, Tadiello A, Trainotti L, Bonghi C, Ziosi V, Costa G (2012) Spermidine application to young developing peach fruits leads to a slowing down of ripening by impairing ripening-related ethylene and auxin metabolism and signaling. Physiol Plantarum 146:86–98

    Article  CAS  Google Scholar 

  • Wu X, Jiang L, Yu M, An X, Ma R, Yu Z (2016) Proteomic analysis of changes in mitochondrial protein expression during peach fruit ripening and senescence. J Proteom 147:197–211

    Article  CAS  Google Scholar 

  • Wu X, Mason M, Yu M, Ma R, Yu Z (2017) Quantitative proteomic analysis of pre- and post-harvest peach fruit ripening based on iTRAQ technique. Acta physiol Plant 39:181

    Article  Google Scholar 

  • Yu JG, Lee GH, Park YD (2012) Physiological role of endogenous S-adenosyl-l-methionine synthetase in Chinese Cabbage. Hortic Environ Biote 53:247–255

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Food Science and Engineering, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Xiaoqin Wu, Chen Huan & Zhifang Yu

  2. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210095, Jiangsu, People’s Republic of China

    Mingliang Yu & Ruijuan Ma

Authors
  1. Xiaoqin Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mingliang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chen Huan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruijuan Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhifang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Author ZY designed the experiment, and XW conducted the experiment and wrote this paper. CH, MY, and RM were participated in part of this experiment and provided some guidance. ZY helped with further revision to the manuscript. All authors read and approved the final version of the manuscript.

Corresponding author

Correspondence to Zhifang Yu.

Additional information

Communicated by M. Stobiecki.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, X., Yu, M., Huan, C. et al. Regulation of the protein and gene expressions of ethylene biosynthesis enzymes under different temperature during peach fruit ripening. Acta Physiol Plant 40, 52 (2018). https://doi.org/10.1007/s11738-018-2628-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-018-2628-5

Keywords

Search

Navigation