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Transcriptome of wax apple (Syzygium samarangense) provides insights into nitric oxide-induced delays of postharvest cottony softening

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Abstract

Wax apple (Syzygium samarangense) is an important tropical fruit crop in Southeast Asia. The rapid cottony softening that occurs after harvest significantly influences the nutrition, flavour and market value of the fruit. Nitric oxide (NO) fumigation has been used to delay the cottony softening process in wax apple; however, the underlying molecular mechanisms at the gene regulation level are poorly understood. In this study, we first sequenced and de novo assembled the wax apple fruit transcriptome using the Illumina Hiseq2000 platform. Meanwhile, we compared the gene expression profiles between NO treatment and control wax apples at different postharvest storage periods using digital gene expression (DGE). This study developed transcriptome data resources for wax apple fruit and provided a foundation for understanding the molecular mechanisms underlying NO-induced cottony softening delay. RNA-Seq generated 5.9 G raw data, which were then de novo assembled into 54,536 unigenes with an N50 length of 1724 bp. 21,433 Unigenes were annotated by the nr database and 3044 were successfully annotated by all databases. The dynamic analysis of the gene expression patterns suggested that NO-induced delays of cottony softening occur in wax apple via the regulation of cell wall degradation, carbohydrate metabolism, oxidation–reduction and plant hormone signal transduction pathways. These results provide a reference for the study of complicated metabolism in non-model perennial species.

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Abbreviations

NO:

Nitric oxide

ROS:

Reactive oxygen species

RNA-Seq:

RNA sequencing

DGE:

Digital gene expression

Pfam:

Protein family

KOG:

euKaryotic Orthologous Groups

GO:

Gene ontology

KEGG:

Kyoto Encyclopaedia of Genes and Genomes

FDR:

False discovery rate

PAL:

Phenylalanine ammonia-lyase

4-CL:

4-Coumarate-CoA ligase

POD:

Peroxidase

SOD:

Superoxide dismutase

CAT:

Catalase

APX:

Ascorbate peroxidase

FFS:

Beta-furan fructosidase

SS:

Sucrose synthetase

ETR:

Ethylene receptor

EBF1_2:

EIN3-binding F-box protein

GST:

Glutathione S-transferase

ADH1_7:

Alcohol dehydrogenase 1/7

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Acknowledgements

We thank Shijun Xiao, Jing Chen, Rui Huang, Xing Wang and Huan Li for helpful experimental guidance.

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Author notes

  1. Yuqi Hao and Zhenzhen Yin contributed equally to this work.

Authors and Affiliations

  1. College of Food and Biological Engineering, Jimei University, 43 Yindou Road, Xiamen, People’s Republic of China

    Fahe Chen, Yuqi Hao, Zhenzhen Yin, Guangbin Wu & Xingxu Jiang

Authors
  1. Fahe Chen
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  2. Yuqi Hao
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  3. Zhenzhen Yin
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  4. Guangbin Wu
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  5. Xingxu Jiang
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Corresponding author

Correspondence to Fahe Chen.

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Availability of data and materials

Raw RNA-Seq reads for the wax apple transcriptome were deposited in the NCBI SRA under the project accession number PRJNA284092 and SRA accession SRR2027825. The raw DGE reads were deposited under the project accession number PRJNA284096 and the corresponding SRA accession nos. SRR2029389, SRR2029390, SRR2029391, SRR2029392, SRR2029393, SRR2029394, SRR2032387, SRR2029395, SRR2029396 and SRR2029397.

Conflict of interest

The authors declare that they have no competing interests.

Funding

This work was supported by grants from the National Natural Science Foundation of China (31171777).

Additional information

Communicated by W. Zhou.

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Chen, F., Hao, Y., Yin, Z. et al. Transcriptome of wax apple (Syzygium samarangense) provides insights into nitric oxide-induced delays of postharvest cottony softening. Acta Physiol Plant 39, 273 (2017). https://doi.org/10.1007/s11738-017-2569-4

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  • DOI: https://doi.org/10.1007/s11738-017-2569-4

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