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Alterations in the proteome of wheat primary roots after wortmannin application during seed germination

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Abstract

Wortmannin is an efficient inhibitor of phosphatidylinositol 3-kinases that is involved in normal plant growth. In this study, we performed the global proteomic analysis of the effects of wortmannin on wheat primary roots during seed germination. Wortmannin significantly inhibited the growth of wheat primary roots in a macroscopic level, and damaged the fine structure of nucleoli and mitochondria in a microscopic level. In total, 75 differentially accumulated protein (DAP) spots representing 66 unique DAPs were identified by linear two-dimensional electrophoresis and tandem mass spectrometry. These DAPs were mainly related to detoxification and defense response, carbon metabolism, and amino acid metabolism. Principal component analysis indicated that wortmannin significantly induced the proteomic changes between the treatment and control groups. Protein–protein interaction analysis revealed a complex network centralized by the 14-3-3 protein which was significantly upregulated in both transcriptional and translational levels. Furthermore, some key DAPs, such as heat shock-related proteins, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, 5-methyltetrahydropteroyltriglutamate–homocysteine methyltransferase, and elongation factor, also showed significantly upregulated accumulation, indicating their key roles in cell homeostasis maintenance, energy supply, and protein biosynthesis. Results obtained from this study provided a new insight into the molecular mechanism of wheat primary root in response to wortmannin during seed germination.

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Abbreviations

2-DE:

Two-dimensional electrophoresis

CAT:

Catalase

DAP:

Differentially accumulated protein

DFC:

Dense fibrillar component

EF:

Eongation factor

FC:

Fibrillar centers

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

GC:

Granular component

HSP:

Heat shock-related protein

GO:

Gene ontology

MALDI–TOF/TOF–MS:

Matrix-assisted laser desorption ionization/time of flight/tandem mass spectrometry

MAPK:

Mitogen-activated protein kinase

NOX:

NADPH oxidase

Nv:

Nucleolar vacuole

PCA:

Principal component analysis

PI3K:

Phosphatidylinositol 3-kinases

PI3P:

Phosphatidylinositol 3-phosphate

PI4P:

Phosphatidylinositol 4phosphate

PPI:

Protein–protein interaction

PtdIns:

Phosphatidylinositol

RBOH:

Respiratory burst oxidase homologue

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TCA:

Tricarboxylic acid

TEM:

Transmission electron microscope

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC, Grant no. 30971453). We are grateful to Dr. Hongjie Li for his assistance with English language and grammatical editing of the manuscript.

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Correspondence to Yueming Yan or Feixiong Zhang.

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This research had no potential conflicts of interest and did not involve any human participants or animals.

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Communicated by M. Stobiecki.

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Cao, H., Ni, X., Zhang, C. et al. Alterations in the proteome of wheat primary roots after wortmannin application during seed germination. Acta Physiol Plant 39, 223 (2017). https://doi.org/10.1007/s11738-017-2511-9

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  • DOI: https://doi.org/10.1007/s11738-017-2511-9

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