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Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock ‘Gisela®5’

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Abstract

The present study investigated the effects of gas-tight and gas-permeable culture containers and different sucrose concentrations, as well as sucrose and mannitol combinations on the development of an effective in vitro slow growth storage protocol (at 4 °C, in darkness) for ‘Gisela®5’ shoot cultures. ‘Gisela®5’ is the most widely used cherry rootstock in Europe. This dwarf triploid hybrid has many advantages over the conventional cherry rootstocks. Optimizations for the cold storage of ‘Gisela®5’ in vitro shoot cultures included use of storage medium supplemented with 10, 20, 30, 45, and 60 g L−1 sucrose and sucrose (15, 30 g L−1) and mannitol (15 g L−1) combinations, contained in gas-tight glass jars and gas-permeable ‘Star Pac™’ bags. Cold storage was prolonged to 12 months, during which in every 3 months, cultures were evaluated. Possibility of 16 month-cold storage in gas-tight glass jars was also explored, during which gas chromatographic analysis was performed for the detection of CO2 and ethylene accumulation for the first 5 months of cold storage. Our results showed that both the 12- and 16-month conservations were possible, especially when 45 or 60 g L−1 sucrose was supplemented to storage medium, contained in glass jars. Mannitol inclusion to the storage medium was also effective to reduce the metabolic activity of the shoot cultures during storage; however, it did not have a significant positive influence on shoot quality in post-conservation.

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Abbreviations

BA:

N 6-benzyladenine

RGR:

Relative growth rate (index)

RGRC :

Relative growth rate of shoot cultures during conservation

RGRR :

Relative growth rate of the cultures recovered during post-conservation

RGRS :

Relative growth rate of the cultures at the multiplication phase in the standard culture conditions

WM:

Workable material

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Acknowledgements

The ‘Ente Cassa di Risparmio of Florence’ is gratefully acknowledged for a 36-month grant to Elif Aylin Ozudogru.

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Authors and Affiliations

  1. CNR-IVALSA, Trees and Timber Institute, via Madonna del Piano 10, Sesto Fiorentino, 50019, Florence, Italy

    Elif Aylin Ozudogru, Carla Benelli & Maurizio Lambardi

  2. Vivai Piante Battistini Società Agricola, 47023, Martorano di Cesena, Forlì-Cesena, Italy

    Giuliano Dradi

Authors
  1. Elif Aylin Ozudogru
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  2. Carla Benelli
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  3. Giuliano Dradi
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  4. Maurizio Lambardi
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Correspondence to Elif Aylin Ozudogru.

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Communicated by J. Van Staden.

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Ozudogru, E.A., Benelli, C., Dradi, G. et al. Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock ‘Gisela®5’. Acta Physiol Plant 39, 94 (2017). https://doi.org/10.1007/s11738-017-2372-2

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  • DOI: https://doi.org/10.1007/s11738-017-2372-2

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