Abstract
The effects of different CdCl2 concentrations on the growth and on certain biochemical parameters of almond seedlings (Prunus dulcis) were studied under controlled conditions in the nutrient solutions containing increasing CdCl2 concentrations ranging from 0 to 150 μM CdCl2. Under Cd stress conditions, damage was variable. Cadmium reduced dry matter production in leaves and roots. While chlorophyll content was severely decreased, that of leaf sugars appeared to be increased. Furthermore, leaf nutritional status seemed to be more altered than that of roots. Both in roots and leaves, there was an increase in MDA content as metal concentration increased. It may be suggested from the present study that toxic concentrations of Cd cause oxidative damage as shown by the increase of lipid peroxidation.
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References
Agrawal M, Singh D (2003) Physiological and biochemical and SO2, singly and in combination. Environ Pollut 121:189–197
Ashwell G (1957) Colorimetric analysis of sugars. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, 3rd edn. Academic, London, pp 85–86
Baccouch S, Chaoui A, El Ferjani E (1998) Nickel toxicity: effects on growth and metabolism of maize. J Plant Nutr 21:577–588
Barceló J, Poschenrieder C (1990) Plant water relations as affected by heavy metal stress: a review. J Plant Nutr 13:1–37
Ben Youssef N, Nouairi I, Temime S, Taamalli W, Zarrouk M, Ghorbel MH (2005) Effets du cadmium sur le métabolisme des lipides de plantules de colza (Brassica napus L.). Comptes Rendus Biologies 328:745–757
Bazzaz FA, Rolfe GL, Carlson RW (1992) Effect of cadmium on photosynthesis and transpiration of excised leaves of corn and sunflower. Physiol Plant 32:373–377
Chaoui A, Mazhoudi S, Ghorbal E, El Ferjani E (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L..). Plant Sci 127:139–147
Chien HF, Kao CH (2000) Accumulation of ammonium in rice leaves in response to excess cadmium. Plant Sci 156:111–115
Citterio S, Santagostino A, Fumagalli P, Prato N, Ranalli P, Sgorbati S (2003) Heavy metal tolerance and accumulation by Cd, Cr and Ni by Cannabis sativa L. Plant Soil 256:243–252
De Filippis LF, Ziegler H (1993) Effect of sublethal concentrations of zinc, cadmium and mercury on the photosynthetic carbon reduction cycle of Euglena. J Plant Physiol 142:167–172
Di Toppi LS, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130
Dong J, Wu FB, Zhang GP (2005) Effect of cadmium on growth and photosynthesis of tomato seedlings. J Zhejinag Univ Sci B6 10:974–980
Drazkiewicz M, Baszynski T (2005) Growth parameters and photosynthetic pigments in leaf segments of Zea mays exposed to cadmium, as related to protection mechanisms. J Plant Physiol 162(9):1013–1021
Dubey RS (1997) Photosynthesis in plants under stressful conditions. In: Pessarakli M (ed) Handbook of photosynthesis. Marcel Dekker, New York, pp 859–875
Dubey RS, Singh AK (1999) Salinity induced accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants. Biol Plant 42:233–239
Foyer C (1988) Feedback inhibition of photosynthesis through source-sink regulation in leaves. Plant Physiol Biochem 26:483–492
Gabrielli R, Pandolfini T, Vergano O, Palandri MR (1990) Comparison of two serpentine species with different nickel tolerance strategies. Plant Soil 122:671–693
Greger M, Lindberg S (1987) Effects of Cd and EDTA on young sugar beet (Beta vulgaris). II. Net uptake and distribution of Mg, Ca and Fe(II)/Fe(III). Physiol Plantarum 69:81–86
Gussarson M, Asp H, Adalststeinsson S, Jensen P (1996) Enhancement of cadmium effects on growth and nutrient composition of birch (Betula pendula) by buthionine sulfoximine (BSO). J Exp Bot 47:211–215
Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–12
Hegedüs A, Erdei S, Janda T, Toth E, Horvath G, Dubits D (2004) Transgenic tobacco plants over producing alfafa aldose/aldehyde reductase show higher tolerance to low temperature and Cadmium stress. Plant Sci 166:1329–1333
Jha AB, Dubey RS (2004) Carbohydrate metabolism in growing rice seedling under arsenic toxicity. J Plant Physiol 161:867–872
Jianguo L, Kunquan L, Jiakuan X, Jiansheng L, Xiaolong L, Jianchang Y, Qingsen Z (2003) Interaction of Cd and five mineral nutrients for uptake and accumulation in different rice cultivars and genotypes. Field Crop Res 83:271–281
Kim CG, Bell JNB, Power SA (2003) Effects of soil cadmium on Pinus sylvestris L. seedlings. Plant Soil 257:443–449
Krupa Z, Baranowska M, Orzol D (1996) Can anthocyanins be considered as heavy metal indicator in higher plants? Acta Physiol Plant 18:147–151
Lagriffoul A, Macquot B, Mench M, Vangronsveld J (1998) Cadmium toxicity effects on growth, mineral and chlorophyll contents, and activities stress related enzymes in young maize plants (Zea mays L.). Plant Soil 200(2):241–250
Moran R, Porath D (1980) Chlorophyll determination in intact tissues using N, N-dimethyl formamide. Plant Physiol 65:478–479
Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Ghorbel MH Zarrouk M (2006) Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus “leaves”. Plant Sci 170:511–519
Padmaja K, Prasad DDK, Prasad ARK (1990) Inhibition of chlorophyll synthesis in Phaseolus vulgaris L. seedlings by cadmium acetate. Photosynthetica 24:399–405
Powell MJ, Davis MS, Francis D (1986) The influence of zinc on the cell cycle in the root meristem of a zinc-tolerant and non tolerant cultivar of Frestuca rubra L. New Phytol 102:419–428
Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115-2126
Sharma SS, Kaul S, Metwally A, Goyal KC, Finkemeier I, Dietz KJ (2004) Cadmium toxicity to barley (Hordeum vulgare) as affected by varying Fe nutritional status. Plant Sci 166:1287–1295
Skorsinska E, Urbanik-Sypniewska T, Russa R, Baszynski T (1991) Galactolipase activity of chloroplasts in cadmium-treated runner bean plants. J Plant Physiol 138:454–459
Somashekaraiah B, Padmaja K, Prasad A (1992) Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris) : Involvement of lipid peroxides in chlorophyll degradation. Physiol Plant 85:85–89
Van Assche F, Clijsters H (1990) Effects of metals on enzyme activity in plants. Plant Cell Environ 13:195–206
Vassilev A (2002) Physiological and agroecological aspects of Cadmium interactions with barley plants: an overview. J Cent Eur Agric 4(1):65–74
Vassilev A, Tordanov T, Tsonev I (1997) Effect of Cd2+ on the physiological state and photosynthetic activity of young barley plants. Photosynthetica 34:293–302
Welch RM, Norvell WA, Schaefer SC, Shaff JF, Kochian LY (1993) Induction of iron III and copper II reduction in pea (Pisium sativum L.) root by Fe and Cu status: does the root-cell plasmalemma Fe III-chelate reductase perform a general role in regulation cations uptake. Planta 190:555–561
Wu FB, Zhang GP (2002) Genotypic variation in kernel heavy metal concentrations in barley and as affected by soil factors. J Plant Nutr 25:1163–1173
Zhang XZ (1992) The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system. In: Zhang XZ (ed) Research methodology of crop physiology. Agriculture Press, Beijing, pp 208–211
Zhang GP, Fukami M, Sekimoto H (2002) Influence of cadmium on mineral concentration and yield components in wheat genotypes differing in Cd tolerance at seedling stage. Field Crop Res 4079:1–7
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Support from the Olive Institute of Sfax and the Laboratory of Environmental Sciences (LARSEN) is gratefully acknowledged. We wish to express our appreciation to Drs K. Gargouri and M. Kallel for their collaboration.
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Communicated by W. Horst.
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Nada, E., Ferjani, B.A., Ali, R. et al. Cadmium-induced growth inhibition and alteration of biochemical parameters in almond seedlings grown in solution culture. Acta Physiol Plant 29, 57–62 (2007). https://doi.org/10.1007/s11738-006-0009-y
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DOI: https://doi.org/10.1007/s11738-006-0009-y