Skip to main content
SpringerLink
Log in

Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis

电针对糖尿病胃轻瘫模型大鼠胃电及胃窦ghrelin 的影响

  • Special Topic for 973 Program
  • Published:
Journal of Acupuncture and Tuina Science Aims and scope Submit manuscript

Abstract

Objective

To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP).

Methods

Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat’s blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay (ELISA); the expression of ghrelin mRNA was detected by real-time polymerase chain reaction (RT-PCR).

Results

Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment (all P<0.01); after treatment, the gastric emptying rate of group B was significantly decreased (P<0.01), the migration rates of small intestine in group B, C, D and E were significantly lower (all P<0.01), and the protein content of ghrelin in group C was significantly decreased (P<0.01); the expressions of ghrelin mRNA were significantly increased in group B, C, D and E (all P<0.01), the mean amplitudes of electrogastrogram in group B and D were significantly decreased (both P<0.01). After treatment, compared with group B, the blood glucose of group C was significantly decreased (P<0.05), the gastric emptying rate and small intestine migration rate were significantly increased in group C and E (P<0.05, P<0.01), the small intestinal migration rate was significantly increased in group D (P<0.05), the expression of ghrelin in protein and mRNA in group C was significantly lower (P<0.01), the expression of ghrelin mRNA in group E was significantly lower (P<0.05), and the mean amplitude of electrogastrogram in group C was significantly increased (P<0.05). After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased (P<0.01). After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased (P<0.01).

Conclusion

EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.

摘要

目的

观察电针对糖尿病胃轻瘫(DGP)模型大鼠胃电及胃窦ghrelin的影响。

方法

将50只Sprague-Dawley (SD)大鼠随机分为A组、B组、C组、D组和E组, 每组10 只, A 组为空白对照组, 不接受任何干预; B组、C组、D组 和E组采用单次腹腔注射2%的链脲佐菌素(STZ)并配合8星期高糖高脂饮食建立DGP大鼠模型。B组为模型组,不接受治疗; C组为电针穴位组, 采用电针足三里、梁门和三阴交治疗; D组为电针非穴位组, 采用电针足三里、梁 门及三阴交的对照点治疗; 胃复安对照组大鼠接受1.7%胃复安药液[10 mL/(kg•bw)]灌胃。用血糖仪检测大鼠血糖 值; 以酚红为标记物, 检测大鼠胃排空率, BL-420F生物机能实验系统检测胃电, 酶联免疫吸附测定法(ELISA)检测 胃窦ghrelin含量, 实时荧光定量聚合酶链反应(RT-PCR)检测ghrelin mRNA的表达。

结果

与A组比较, 治疗前和治疗 后B、C、D和E组的血糖均显著增高(均P<0.01); 治疗后B组大鼠胃排空率显著降低(P<0.01), 治疗后B、C、D和E 组小肠推进率显著降低(均P<0.01), 治疗后C组胃窦ghrelin含量显著降低(P<0.01), 治疗后B、C、D和E组胃窦 ghrelin mRNA表达显著升高(均P<0.01), 治疗后B组和D组胃电平均振幅显著降低(均P<0.01); 与B组比较, C组治 疗后血糖明显下降(P<0.05), 治疗后C、E组胃排空率及小肠推进率明显升高(P<0.05, P<0.01), D组小肠推进率明 显升高(P<0.05), 治疗后C组胃窦ghrelin含量及mRNA表达显著降低(P<0.01), E组ghrelin mRNA表达显著降低 (P<0.01), 治疗后C组胃电平均振幅明显增加(P<0.05); 与D组比较, 治疗后C组胃窦ghrelin含量及mRNA表达显著 降低(P<0.01); 与E组比较, 治疗后C组胃窦ghrelin含量显著降低(P<0.01)。

结论

电针足三里、梁门及三阴交可 以调节DGP模型大鼠的血糖水平, 增强胃电活动, 促进胃排空, 调节胃窦ghrelin水平及下调ghrelin mRNA的表达。

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shin AS, Camilleri M. Diagnostic assessment of diabetic gastroparesis. Diabetes, 2013, 62(8): 2667–2673.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Li YH, Wang QG, Chen M, Li LN, Wang D, Zhang DM, Zhong XG, Wang PL. Effect of Banxia Xiexin decoction and its components on coefficient of variability of slow wave electrogastric rhythm in rats with electrogastric dysrhythmia. Zhongguo Zhongxiyi Jiehe Zazhi, 2006, 26 (6): 53–55.

    CAS  PubMed  Google Scholar 

  3. Wang Y, Dong L, Zou BC, Shi HT, Jiang J, Song YH. The effect of ghrelin on the gastrointestinal interdigestive myoelectric complex in rats and the distribution characteristics of its receptor in the digestive tract. Xi’an Jiaotong Daxue Xuebao (Yixue Ban), 2014, 35(2): 186–190.

    Google Scholar 

  4. Jia J, Chang XR, Yan J, Yi SX, Lin YP, Yue ZH, Liu M, Peng Y. Effect of moxibustion on plasma levels of ß-EP, MTL and SS in rats with spleen deficiency. Shijie Huaren Xiaohua Zazhi, 2011, 19(34): 3498–3502.

    Google Scholar 

  5. Li XH, Lin GH, Zou ZC. Effect of electroacupuncture on motilin and somatostatin in rats with diabetes gastroparesis. Shanghai Zhenjiu Zazhi, 2015, 34(9): 97–100.

    Google Scholar 

  6. Sun XF, Cheng WD, Duan YQ, Xing WT, Zhou YY. Research of variation of gastrointestinal hormone, SS, GAS and CCK content in serum and gastric tissue and traditional Chinese medicine intervention of spleen deficiency syndrome rats. Shizhen Guoyi Guoyao, 2014, 25(3): 756–758.

    Google Scholar 

  7. Wu DK, Zheng JH, Tang YM, Mo QZ, Qin WF. Changes in levels of GAS, CCK, and SS in serum and gastric tissue of spleen deficiency rats: effect of traditional Chinese medicine intervention. Shijie Huaren Xiaohua Zazhi, 2015, 23(11): 1791–1795.

    Google Scholar 

  8. Lü FX, Wei LZ, Tian ZB, Wang LH, Kong XJ, Yin JP. Effect of glucagon-like peptide-1 in the dorsal vagal complex on the expression of GLP-1 receptor in the hypothalamus and the gastric emptying in diabetic rats. Weichangbingxue He Ganbingxue Zazhi, 2013, 22(4): 333–336.

    Google Scholar 

  9. Li XM, Wei LZ, Tian ZB, Wang LH, Ding XL, Miao YH. Influence of glucagon-like peptide-1 on non-receptorsmediated pathway of gastric emptying in diabetic rats. Weichangbingxue He Ganbingxue Zazhi, 2014, 23(3): 279–282.

    CAS  Google Scholar 

  10. Yu H, Cheng JP, Zhang DQ, Tang CJ, Huang KY, Tan LJ, Yang SB, Mei ZG. Effect of acupuncture combined with Chinese medicine on the expression of interstitial cells of Cajal, substance P and nerve nitric oxide synthase in diabetic mice with gastroparesis antrum. Shijie Zhenjiu Zazhi, 2015, 25(3): 35–42.

    CAS  Google Scholar 

  11. Lin YP, He FE, Peng Y, Wang QQ, Shen J. Effects of electroacupuncture on gastrointestinal hormones in rats with diabetic gastroparesis. Beijing Zhongyiyao Daxue Xuebao, 2015, 38(12): 847–851.

    CAS  Google Scholar 

  12. He FE, Wang QQ, Lin YP, Peng Y, Shen J. Effect of electroacupuncture on the INS level and CCK content in a rat model of DGP. Shanghai Zhenjiu Zazhi, 2016, 35(1): 81–84.

    Google Scholar 

  13. Lin YP, Wang QQ, Peng Y, He FE, Shen J. Effect of electroacupunctue at Zusanli (ST 36) and others on gastrointestinal motility and expression of ghrelin and growth hormone secretagogue receptor mRNA in diabetic gastroparesis rats. Zhen Ci Yan Jiu, 2015, 40(4): 290–295.

    PubMed  Google Scholar 

  14. Wang QQ, He FE, Lin YP. Observation on indexes related the evolution process of diabetic gastroparesis rat models. Hunan Zhongyiyao Daxue Xuebao, 2014, 34(10): 6–10.

    CAS  Google Scholar 

  15. Li ZR. Experimental Acupuncture Science. Beijing: China Press of Traditional Chinese Medicine, 2007: 327–329.

    Google Scholar 

  16. Peng Y, He FE, Wang QQ, Chen W, Liu Q, Yi SX, Lin YP. Effect of electroacupuncture on ghrelin and GHSR protein and gene expression in gastric antrum of diabetic rats. Zhongguo Zhongyi Jichu Yixue Zazhi, 2016, 22(8): 1088–1091.

    Google Scholar 

  17. Stoian A, Banescu C, Balasa RI, Motataianu A, Stoian M, Moldvoan VG, Voidazan S, Dobreanu M. Influence of GSTM1, GSTT1, and GSTP1 polymorphisms on type 2 diabetes mellitus and diabetic sensorimotor peripheral neuropathy risk. Dis Markers, 2015, 2015: 638693.

    Article  PubMed  PubMed Central  Google Scholar 

  18. Weng JP. The current progress of STRATEGY study. Thirteenth National Conference on Endocrinology of Chinese Medical Association. Chongqing: Chinese Medical Association, Endocrinology Branch of the Chinese Medical Association, 2014: 2.

    Google Scholar 

  19. Xu MY, Lu GH, Chen MD. Diabetes Mellitus. Shanghai: Shanghai Science and Technology Press, 2010: 490–494.

    Google Scholar 

  20. Wu L, Liu CL. Advances in pathogenesis of diabetic gastroparesis. Xiandai Yiyao Weisheng, 2013, 29(14): 2152–2154.

    Google Scholar 

  21. Huang JZ, Wang XW. Treatment of 25 case diabetic gastroparesis by domperidone combined with metoclopramide. Zhongguo Zhongxiyi Jiehe Xiaohua Zazhi, 2013, 21(2): 103–104.

    Google Scholar 

  22. Zhu DN, Wang TH. Physiology. Beijing: People’s Medical Publishing House, 2013: 201–204.

    Google Scholar 

  23. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone releasing acylated peptide from stomach. Nature, 1999, 402(6762): 656–660.

    Article  CAS  PubMed  Google Scholar 

  24. Ma XB, Xu WH. Ghrelin in gastrointestinal diseases. Shijie Huaren Xiaohua Zazhi, 2013, 21(3): 239–243.

    CAS  Google Scholar 

  25. Liang A, Gao HK. The relationship between ghrelin and energy metabolism. Zhongguo Yiyao Kexue, 2012, 2(5): 42–43, 46.

    Google Scholar 

  26. Chen Y, Zheng Y, Shi HL, Fei XY, Yuan JY. Relationship between ghrelin and abnormal gastric emptying in diabetes mellitus. Shijie Huaren Xiaohua Zazhi, 2014, 22(34): 5298–5303.

    CAS  Google Scholar 

  27. Yin H, Zhang XJ, Yan X, Chen M, Liu C. Relationship between ghrelin and gastric emptying in diabetic rats at different stages. Hainan Yixue, 2014, 25(19): 2809–2813.

    CAS  Google Scholar 

  28. Yang L, Wei LZ, Tian ZB, Li YQ. The effect of blood glucose variation on gastric emptying and ghrelin expression in diabetic rats. Zhonghua Xiaohua Zazhi, 2007, 27(4): 221–224.

    CAS  Google Scholar 

  29. Xu L, Guo FF, Sun XR, Gao SL, Gong YL, Qiu BB. Effect of ghrelin on firing activity of gastric distension-sensitive neurons in hypothalamic arcuatus nucleus and gastric motility in diabetic rats. Zhongguo Bingli Shengli Zazhi, 2013, 29(1): 28–35.

    Google Scholar 

  30. Wang LX, Wang QL, Pang MJ, Zhu H, Guo FF, Sun XR, Xu L. Dynamics expression and regulation of ghrelin and nesfatin-1 in diabetic rats. Xiandai Shengwu Yixue Jinzhan, 2015, 15(10): 1820–1824.

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by National Natural Science Foundation of China (国家自然科学基金项目, No. 81403487); National Basic Research Program of China (973 Program, 国家重点基础研究发展计划, No. 2014CB543102); Youth Fund of Hunan Province Education Office (湖南省教育厅青年基金, No. 14B128).

Author information

Authors and Affiliations

  1. Hunan University of Chinese Medicine, Changsha, 410208, China

    Hai-jiao Chen  (陈海交), Ya-ping Lin  (林亚平), Jian-wen Yang  (杨建文), Cheng-cheng Zhang  (张程程), Yan Peng  (彭艳), Wei-wei Liu  (刘薇薇), Li Liu  (刘丽), Mi Liu  (刘密) & Zeng-hui Yue  (岳增辉)

Authors
  1. Hai-jiao Chen  (陈海交)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ya-ping Lin  (林亚平)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jian-wen Yang  (杨建文)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cheng-cheng Zhang  (张程程)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yan Peng  (彭艳)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei-wei Liu  (刘薇薇)
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Li Liu  (刘丽)
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Mi Liu  (刘密)
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Zeng-hui Yue  (岳增辉)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yan Peng  (彭艳).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, Hj., Lin, Yp., Yang, Jw. et al. Effect of electroacupuncture on electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis. J. Acupunct. Tuina. Sci. 15, 242–249 (2017). https://doi.org/10.1007/s11726-017-1008-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11726-017-1008-9

Keywords

关键词

中图分类号

Search

Navigation