Abstract
This study aimed to investigate the effect of LyPB on the intestinal microflora of giant pandas with indigestion, using high-throughput sequencing (HTS) technology. The species distribution and microfloral density and diversity before and after administration of the LyPB probiotic agent were analyzed. LyPB evidently has the ability to adjust the floral imbalance in the panda’s intestine. To test the effects of LyPB on the microflora of the panda gut, fecal samples were taken from a healthy giant panda (Anan) without administration of LyPB and from a dyspeptic giant panda Yangyang before and after LyPB administration. Compared with the sample obtained from healthy Anan (anan-c) and that obtained from dyspeptic Yangyang before LyPB administration (yangyang1), the sample taken from Yangyang (yangyang2) after LyPB administration displayed a significant increase in the operational taxonomic unit index. An increase in the Chao index indicated an increase in the microfloral richness, while an increase in the Shannon index indicated an increase in microfloral diversity. At phylum and genus levels, a significant increase was observed in the density of probiotic bacteria of phylum firmicutes, genus Streptococcus, while a drastic reduction in the density of Escherichia coli/Escherichia coli Shigella/bacteria of genus Shigella was observed. Data obtained in this study shows that LyPB preparations successfully improve the microbial structure within the panda’s intestinal canal by significantly increasing the effective microbial community and decreasing the number of pathogenic microbes.
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References
Bose S, Han KW, Lee MJ, Kim H (2013) Intestinal protective effects of herbal-based formulations in rats against neomycin insult. Evid Based Complement Altern Med eCAM. https://doi.org/10.1155/2013/16128
Che Othman SF, Noor Aziah AA, Ahmad R (2012) Effects of jackfruit puree supplementation on lactic acid bacteria (Lactobacillus acidophilus FTDC 1295) in terms of viability and chemical compositions of dadih. Int Food Res J 19(4):1687–1697
Dahiya JP, Hoehler D, Wilkie DC, Van Kessel AG, Drew MD (2005) Dietary glycine concentration affects intestinal Clostridium perfringens and lactobacilli populations in broiler chickens. Poult Sci 84(12):1875–1885
Gaon RD, Garcia H, Winter L, Rodríguez N, Quintás R, González SN, Oliver G (2003) Effect of Lactobacillus strains and Saccharomyces boulardii on persistent diarrhea in children. Medicina 63(4):293–298
Guandalini S, Pensabene L, Zikri MA, Dias J, Casali L, Hoekstra H, Kolacek S, Massar K, Micetic-Turk D, Papadopoulou A, Sousa JD, Sandhu B, Szajewska H, Weizman Z (2000) Lactobacillus GG administered in oral rehydration solution to children with acute diarhea: a multicenter European trial. J Pediatr Gastroenterol Nutr 30(1):54–60. https://doi.org/10.1097/00005176-200001000-00018
He YG, Jin L, Li G, Li CW, Li B et al (2017) Gut microbiome of adult giant pandas based on high-throughput sequencing technology. Chin J Appl Environ Biol 23(5):771–777. https://doi.org/10.3724/SP.J.1145.2016.05023
Kale VV, Trivedi RV, Wate SP, Bhusari KP (2005) Development and evaluation of a suppository formulation containing Lactobacillus and its application in vaginal diseases. N Y Acad Sci 1056:359–365. https://doi.org/10.1196/annals.1352.017
Li YD, Shimizu T, Hosaka A, Kaneko N, Ohtsuka Y, Yamashiro Y (2004) Effects of bifidobacterium breve supplementation on intestinal flora of low birth weight infants. Pediatr Int 46:509–515
Lin XF, Yin WZ, Zhang Z, Ma JZ, Li J (2017) Optimization of fermentation conditions for Bifidobacrium animalis production on design-expert. Food Res Dev 38(4):144–148. https://doi.org/10.3969/j.issn.1005-6521.2017.07.20
Lyra A, Hillilä M, Huttunen T, Männikkö S, Taalikka M, Tennilä J, Tarpila A, Lahtinen S, Ouwehand AC, Veijola L (2016) Irritable bowel syndrome symptom severity improves equally with probiotic and placebo. World J Gastroenterol 22(48):10631–10642. https://doi.org/10.3748/wjg.v22.i48.10631
McFarland LV, Surawicz CM, Greenberg RN et al (1994) A randomized placebo-controlled Trial of Saccharomyces boulardii in combination with standard antibiotics for clostridium difficile disease. Jam Med Assoc 271(24):1913–1918
Muralidhara KS, Sheggeby GG, Elliker PR, England DC, Sandine WE (1977) Effect of feeding Lactobacilli on the coliform and Lactobacillus flore of intestinal tissue and feces from piglets. J Food Prot 40(5):288–295. https://doi.org/10.4315/0362-028x-40.5.288
Öhman L, Simrén M (2013) Intestinal microbiota and its role in irritable bowel syndrome (IBS). Curr Gastroenterol Rep 15:323. https://doi.org/10.1007/s11894-013-0323-7
Qu W, Zhang Z, Ma JZ, Liu H, Ya N (2017) Effect of probiotics on gut microbiota in mice evaluated by high-throughput sequencing. Food Sci 38(1):214–219. https://doi.org/10.7506/spkx1002-6630-201701036
Sanders ME, Kleanhammer TR (2001) Invited review: The scientific basic of Lactobacillus acidophilus NCFM functionality as a probiotic. J Dairy Sci 84(2):319–331. https://doi.org/10.3168/jds.s0022-0302(01)74481-5
Sek H, Shiohara M, Matsumura T et al (2003) Prevention of antibiotic-associated diarrhea in children by Clostridium butyricum MIYAIRI. Pediatr Int 45(1):86–90. https://doi.org/10.1046/j.1442-200X.2003.01671.x
Standen BT, Rodiles A, Peggs DL, Davies SJ, Santos GA, Merrifield DL (2015) Modulation of the intestinal microbiota and morphology of tilapia, Oreochromis niloticus, following the application of a multi-species probiotic. Appl Microbiol Biotechnol 99(20):8403–8417. https://doi.org/10.1007/s00253-015-6702-2
Vargas-Albores F, Porchas-Cornejo MA, Martínez-Porchas M, Villalpando-Canchola E, Gollas-Galván T, Martínez-Córdova LR (2017) Bacterial biota of shrimp intestine is significantly modified by the use of a probiotic mixture: a high throughput sequencing approach. Helgol Mar Res 71:5. https://doi.org/10.1186/s10152-017-0485-z
Wagner R, Balish E (1998) Potential hazards of probiotic bacteria for immunodeficient patients. Bull Inst Pasteur 96(4):165–170. https://doi.org/10.1016/S0020-2452(98)80011-0
Wei FW, Wang ZW, Feng ZJ, Li M, Zhou A (2000) Seasonal energy utilization in bamboo by the red panda (Ailurus fulgens). Zoo Biol 19(1):27–33
Yan TT, Ran JH, Zhao CH, Zhong X, Liang MY (2017) Climate-change impacts on bamboo distribution and giant panda habitat in Qionglai Mountains. Acta Ecol Sin 37(7):2360–2367. https://doi.org/10.5846/stxb201512032424
Yang SY, Pei WJ, Sun FL, Chen X, Wu MY, Shi XQ, Xm Wu, Ren JS, Jia KS (2004) Feces-based detection of Yersinia enterocolitica in intestine of Ailuropoda melanoleuca by PCR. Acta Theriol Sin 24(2):182–184. https://doi.org/10.3969/j.issn.1000-1050.2004.02.015
Zhang Z, Yin WZ, Ya N, Ma JZ (2017) Screening of celluloytic bacterium from giant panda’s feces and optimization of condition for cellulase production. Chin J Anim Nutr 29(8):2817–2825. https://doi.org/10.3969/j.issn.1006-267x.2017.08.026(in Chinese)
Zhu LF, Yang ZS, Yao R, Xu LL, Chen H, Gu XD, Wu TG, Yang XY (2018) Potential mechanism of detoxification of cyanide compounds by gut microbiomes of bamboo-eating pandas. MSphere. https://doi.org/10.1128/msphere.00229-18
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Project funding: This research was supported by International Giant Panda Research Fund of State Forestry Administration (SG1409).
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Corresponding editor: Yu Lei.
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Zhang, Y., Zhang, Z., Ma, J. et al. High-throughput sequencing analysis of the regulation of intestinal flora in giant pandas with indigestion using a probiotic agent LyPB. J. For. Res. 31, 2589–2595 (2020). https://doi.org/10.1007/s11676-020-01171-1
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DOI: https://doi.org/10.1007/s11676-020-01171-1