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Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ‘Dalecartica’

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Abstract

Auxin polar transport genes PIN (PIN-FORMED) determine the concentration gradient of auxin in plants. To understand the relationship between the development of different tissues in Betula pendula ‘Dalecartica’, BpPIN gene expression and indole-3-acetic acid (IAA) content were analyzed using qRT-PCR, ELISA, and GUS staining. Gene expression of BpPIN genes and IAA levels in the leaves, buds, stems, xylem, and roots of B. pendula ‘Dalecartica’ and B. pendula as a control were measured. BpPIN1, BpPIN5 and BpPIN6 were upregulated during development in both species, suggesting a dominant role in the development of B. pendula ‘Dalecartica’ leaves. Moreover, BpPIN1 gene expression was positively associated with IAA levels during leaf, vein and petiole development in B. pendula ‘Dalecartica’ only. The correlation coefficient of the first three leaves was 0.69 (P = 0.04), while that of the first three petioles was 0.85 (P = 0.001). In addition, GUS staining of the pro-DR5::GUS transgenic line of cultivar was correlated with the results of BpPIN1 expression. Overall, these findings suggest that BpPIN1 is associated with the formation of lobed leaves in B. pendula ‘Dalecartica’.

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, People’s Republic of China

    Chang Qu, Xiuyan Bian, Rui Han, Jing Jiang & Guifeng Liu

  2. University of Florida, Lake Alfred, FL, USA

    Qibin Yu

Authors
  1. Chang Qu
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  2. Xiuyan Bian
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  3. Rui Han
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  4. Jing Jiang
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  5. Qibin Yu
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  6. Guifeng Liu
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Corresponding author

Correspondence to Guifeng Liu.

Additional information

Project funding: The work was supported by the National Natural Science Foundation of China (NSFC; Grant No. 31670673) and the 111 Project of China (Grant No. B16010).

The online version is available at http://www.springerlink.com

Corresponding editor: Tao Xu.

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Qu, C., Bian, X., Han, R. et al. Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ‘Dalecartica’. J. For. Res. 31, 87–97 (2020). https://doi.org/10.1007/s11676-018-0865-5

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  • DOI: https://doi.org/10.1007/s11676-018-0865-5

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