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Establishment of a micropropagation supporting technology for the Fraxinus mandshurica × Fraxinus sogdiana

  • Plant Tissue Culture
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Abstract

The interspecific hybridization can take advantage of heterosis and combine the double parental traits most extensively, and is an effective strategy for improving species and stress tolerance breeding for cross-pollinated trees. Species of Fraxinus L. are important for landscaping, timber, and afforestation tree species. To solve problems of poor cold adaption associated with the introduction of Fraxinus sogdiana in Maoershan (Heilongjiang province, Northeast China), to improve pest resistance of Fraxinus mandshurica, and to take advantages of the characteristics of F. mandshurica and F. sogdiana, the interspecific hybridization of F. mandshurica × F. sogdiana was conducted. Tissue culture is the key technology for the promotion of excellent clones and is a powerful tool for germplasm conservation of endangered or valuable plant species. Basing on the embryo culture of hybridization seeds, a complete and efficient micropropagation technology of the Fraxinus mandshurica × Fraxinus sogdiana for its production was established by the uniform design, regression analysis, and verification tests for optimal conditions. In this micropropagation technology, induction rates of callus, adventitious shoot, and adventitious root were 83.5%, 65.9%, and 80.0%, respectively; the number of adventitious shoots was 12.5 produced per callus and the survival rate of the seedlings grew in the greenhouse could be 91.3% after acclimatization. These findings enriched the germplasm resources of Fraxinus species, supplied a micropropagation supporting technology for production of F1 hybrids with ideal traits and accelerated the genetic crossbreeding process of F. mandshurica.

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Abbreviations

PGRs:

Plant growth regulators

WPM:

Woody plant medium

IBA:

Indole-3-butyric acid

6-BA:

6-Benzyladenine

TDZ:

Thidiazuron

NAA:

1-Naphthlcetic acid

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Funding

This study was funded by the Applied Technology Research and Developmental Plan Program of Heilongjiang Province (GA19B201) and Independent Innovation Foundation for Doctoral students of Northeast Forestry University (2572015AA11).

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Authors and Affiliations

  1. Key Laboratory of Saline-Alkaline Vegetation Ecology Restoration (SAVER), Ministry of Education, Northeast Forestry University, Harbin, 150040, China

    Liming He, Fansuo Zeng, Lei Yu & Yaguang Zhan

  2. Department of Forest Bioengineering, College of Life Science, Northeast Forestry University, Harbin, 150040, China

    Liming He, Yungang Xu, Fansuo Zeng, Lei Yu & Yaguang Zhan

  3. Forest Botanical Garden of Heilongjiang Province, Harbin, 150040, China

    Hongmei Tian

  4. Shanhetun Forestry Bureau, Wuchang City, Harbin, 150200, China

    Ying Xiao & Hualing Liu

Authors
  1. Liming He
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  2. Yungang Xu
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  3. Fansuo Zeng
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  4. Hongmei Tian
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  5. Ying Xiao
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  6. Hualing Liu
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  7. Lei Yu
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  8. Yaguang Zhan
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Correspondence to Yaguang Zhan.

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Editor: Yong Eui Choi

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He, L., Xu, Y., Zeng, F. et al. Establishment of a micropropagation supporting technology for the Fraxinus mandshurica × Fraxinus sogdiana. In Vitro Cell.Dev.Biol.-Plant 57, 307–318 (2021). https://doi.org/10.1007/s11627-021-10157-5

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  • DOI: https://doi.org/10.1007/s11627-021-10157-5

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