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Cedrol alleviates the apoptosis and inflammatory response of IL-1β-treated chondrocytes by promoting miR-542-5p expression

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Abstract

Cedrol has been shown to exert anti-tumor, anti-inflammatory, and anti-oxidative effects, but its role in osteoarthritis (OA) is unclear. This study aimed to explore the effect of cedrol in OA. Chondrocytes were isolated from newborn rats and cultured in Dulbecco’s modified Eagle’s medium (DMEM). Then, Alcian blue staining was used to identify the chondrocytes. IL-1β and cedrol were used to treat chondrocytes. Cell viability and apoptosis were measured by MTT and flow cytometry assays, respectively. The expressions of miR-542-5p, miR-26b-5p, miR-572, miR-138-5p, miR-328-3p, miR-1254, Bcl-2, Bax, iNOS, COX-2, and MMP-13 were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or western blot. NO and PGE2 levels were detected by ELISA. All the cells extracted from the newborn rats were dyed blue, indicating that the cells were chondrocytes. IL-1β could reduce the viability and promote apoptosis and inflammatory response of chondrocytes, while cedrol could reverse the effect of IL-1β. In addition, cedrol could significantly increase the expression of miR-542-5p in IL-1β-treated chondrocytes. Moreover, miR-542-5p inhibitor could partly reverse the effect of cedrol in the apoptosis and inflammation response of chondrocytes. Cedrol alleviated IL-1β-induced apoptosis and inflammatory response of chondrocytes by promoting miR-542-5p expression.

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Funding

This work was supported by the 2019 Nanjing Municipal Health Technology Development Special Fund Project [ZKX19036].

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  1. Wangchao Dong and Shanshan Wang contributed equally to this work

Authors and Affiliations

  1. Department of Orthopedics, Nanjing Hospital of Chinese Medicine, Nanjing, China

    Wangchao Dong, Weiqing Qian & Suming Li

  2. Department of Pharmacy, Children’s Hospital of Nanjing Medical University, Nanjing, China

    Shanshan Wang

  3. Department of Orthopedics, Jiangsu Province Hospital of Chinese Medicine, Qinhuai District, 155 Hanzhong Road, Nanjing, 210000, Jiangsu Province, China

    Peimin Wang

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Dong, W., Wang, S., Qian, W. et al. Cedrol alleviates the apoptosis and inflammatory response of IL-1β-treated chondrocytes by promoting miR-542-5p expression. In Vitro Cell.Dev.Biol.-Animal 57, 962–972 (2021). https://doi.org/10.1007/s11626-021-00620-3

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