Abstract
Yangtze sturgeon (Acipenser dabryanus) is an endangered endemic freshwater fish of China. Cell-line is a potential means used for long-term preservation of germplasm resources and an ideal in vitro model in place of living organisms for biological studies. Here, culture condition and characterization of fin-derived cell in Yangtze sturgeon were carried out. Tissue explant techniques have been efficiently used in the Yangtze sturgeon caudal fin (YSCF) culture. The YSCF cell line showed a fibroblast-like morphology and stable growth in minimum essential medium eagle’s (MEME) supplemented with 10–20% fetal bovine serum at 25°C. Cells were cryopreserved with preservative DMSO in liquid nitrogen and grew normally after recovery. No bacterial, fungal, or mycoplasma contamination was detected in the YSCF cells. Karyotype analysis of the YSCF cells showed that the chromosome numbers of the YSCF ranged from 242 to 273, and the modal chromosome number was identified as 264 at passage 9. The YSCF cells were confirmed from A. dabryanus by assay of 16S rRNA and COI. Furthermore, GFP reporter gene was successfully transferred into YSCF cells and expressed. The established YSCF cell lines will contribute to the preservation of germplasm resources and provide a useful vitro tool for further biological studies in sturgeon species.
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Acknowledgments
We thank Dezhi Zhang, Jun Rao, and Liu Yong for providing help in the fish breeding and sample preparation.
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This work was supported by the Three Gorges Environmental Funds of China Three Gorges Corporation (XN270).
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The study was carried out in accordance with the guidelines and regulations of the National Institute of Health Guide for the Care and Use of Laboratory Animals and was approved by the Institutional Review Board on Bioethics and Biosafety of the Chinese Sturgeon Research Institute.
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Liu, J., Liu, X., Zeng, Q. et al. Establishment and characterization of a cell line derived from fin of the endangered Yangtze sturgeon (Acipenser dabryanus). In Vitro Cell.Dev.Biol.-Animal 56, 650–658 (2020). https://doi.org/10.1007/s11626-020-00488-9
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DOI: https://doi.org/10.1007/s11626-020-00488-9