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Construction of a highly sensitive NADH sensing platform based on PDDA-rGO nanocomposite modified electrode

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Abstract

In this article, we constructed an electrochemical sensor for highly sensitive determination of β-nicotinamide adenine dinucleotide (NADH) using the poly(diallyldimethylammonium chloride)-functionalized reduced graphene oxide nanocomposite modified electrode (PDDA-rGO/GCE). The nanocomposite was characterized by transmission electron microscopy, Fourier transform-infrared spectrometry, ultraviolet-visible absorption spectrometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental results demonstrated that the fabricated sensor exhibited excellent electrocatalytic activity on the oxidation of NADH with a decreased about 200 mV in overpotential and 5.0-fold increment in the oxidation peak current compared to a bare GCE. Applying cyclic voltammetry, a wide linear range from 1.0 × 10−7 to 2.9 × 10−3 M with low limit of detection (LOD) of 3.4 × 10−8 M and high sensitivity of 463.3 μA mM−1 cm−2 were obtained, which was superior to other NADH electrochemical sensors reported previously. Furthermore, the fabricated NADH sensor possessed excellent selectivity, reproducibility, and stability. The oxidation mechanism of NADH at the PDDA-rGO/GCE was also discussed in detail.

A simple NADH sensing platform based on PDDA-rGO composite is constructed, which exhibits an ultra-low detection limit ( 3.4×10-8 M) towards the oxidation of NADH, and the electrochemical reaction mechanism of NADH is discussed in detail.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 21565021 and 21245004).

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  1. Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, No. 967 Anning East Road, Lanzhou, Gansu, 730070, People’s Republic of China

    Juanjuan Lu, Yuelin Liu, Xinhe Liu, Xiaoquan Lu & Xiuhui Liu

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  1. Juanjuan Lu
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  2. Yuelin Liu
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  3. Xinhe Liu
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  4. Xiaoquan Lu
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Correspondence to Xiuhui Liu.

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ESM 1

The study of NADH sensor at different scan rates, the optimization of experimental variables, and the study of reproducibility and stability of the modified electrode. (DOC 4478 kb)

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Lu, J., Liu, Y., Liu, X. et al. Construction of a highly sensitive NADH sensing platform based on PDDA-rGO nanocomposite modified electrode. Ionics 22, 2225–2233 (2016). https://doi.org/10.1007/s11581-016-1753-7

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  • DOI: https://doi.org/10.1007/s11581-016-1753-7

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