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Fingerprinting of Potato Genotypes from Estonian Genebank Collection Using SSR Markers

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Abstract

Over 450 potato varieties and landraces are preserved as in vitro cultures by the Plant Biotechnology department of Estonian Crop Research Institute. This collection contains germplasm from various countries as well as old Estonian landraces. These potato accessions are periodically grown in the field for verification; however, this is not always sufficient to validate varieties and, in many cases, the true origin or identity of old landraces is not known. A reliable and cost-effective method for fingerprinting Estonian potato germplasm needed to be implemented. Simple sequence repeat (SSR) markers are known as robust and efficient markers for the identification of tetraploid potato. Here, fingerprinting results are presented for 103 genotypes from the Estonian potato collection using 8 SSR markers. It was shown that as few as three markers were sufficient to yield unique fingerprints for the given set of samples. SSR markers were used to estimate the allele copy number with varying success. Additionally, the reproducibility of four of these markers was assessed in two different laboratories, and repeatable results were observed. It was verified that the putative landrace ‘Kuldkera’ has the same genetic fingerprint as the variety ‘Ando’, whilst an expected clone of ‘Early Rose’ is a completely different genotype. Implementation of genetic fingerprinting and these results is of high importance for further potato genebank management and Estonian breeding programme.

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Acknowledgements

The authors would like to thank Karmen Tõnismann for testing SSR primers as a part of her Master Thesis, Pille Veidenberg, Jekaterina Aida and Susete Ulliel for their contribution to the laboratory work, as well as Erkki Truve and Kadri Järve for their highly appreciated professional advice.

Availability of Data and Material

Supplementary figures and tables are provided with the article.

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Not applicable.

Funding

Funding for this project was provided by Estonian national programme ‘Collection and Conservation of Plant Genetic Resources for Food and Agriculture in 2014–2020’ by the Ministry of Rural Affairs of the Republic of Estonia.

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Authors and Affiliations

  1. Estonian Crop Research Institute, J. Aamisepa 1, 48309, Jõgeva, Jõgeva Vald, Estonia

    Anna Ivanova-Pozdejeva, Agnes Kivistik, Liisa Kübarsepp, Terje Tähtjärv, Aide Tsahkna & Kristiina Laanemets

  2. Plant Breeding and Genetic Resources, Agroscope, Nyon, Switzerland

    Eric Droz

  3. Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, 51014, Tartu, Estonia

    Liisa Kübarsepp

Authors
  1. Anna Ivanova-Pozdejeva
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  2. Agnes Kivistik
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  3. Liisa Kübarsepp
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  4. Terje Tähtjärv
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  5. Aide Tsahkna
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  6. Eric Droz
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  7. Kristiina Laanemets
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Contributions

Study conception and design: Anna Ivanova-Pozdejeva, Kristiina Laanemets. Material preparation: Liisa Kübarsepp (in vitro plants), Terje Tähtjärv and Aide Tsahkna (potato tubers), and Eric Droz (sample preparation for SSR analysis at Agroscope, Switzerland). Formal analysis and investigation: Anna Ivanova-Pozdejeva, Agnes Kivistik, Kristiina Laanemets, and Eric Droz (SSR analysis at Agroscope, Switzerland). Original draft preparation: Anna Ivanova-Pozdejeva, Kristiina Laanemets. Review and editing: all authors contributed to the manuscript review and editing.

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Correspondence to Anna Ivanova-Pozdejeva.

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Ivanova-Pozdejeva, A., Kivistik, A., Kübarsepp, L. et al. Fingerprinting of Potato Genotypes from Estonian Genebank Collection Using SSR Markers. Potato Res. 65, 153–170 (2022). https://doi.org/10.1007/s11540-021-09514-z

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