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Effects of ethyl acetate extract of Kaempferia parviflora on brown adipose tissue

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Abstract

We have previously reported the effects of Kaempferia parviflora (KP), including anti-obesity, preventing various metabolic diseases, and regulating differentiation of white adipose cells. In this study we used Tsumura, Suzuki, Obese Diabetes (TSOD) mice—an animal model of spontaneous obese type II diabetes—and primary brown preadipocytes to examine the effects of the ethyl acetate extract of KP (KPE) on brown adipose tissue, which is one of the energy expenditure organs. TSOD mice were fed with MF mixed with either KPE 0.3 or 1 % for 8 weeks. Computed tomography images showed that whitening of brown adipocytes was suppressed in the interscapular tissue of the KPE group. We also examined mRNA expression of uncoupling protein 1 (UCP-1) and β3-adrenalin receptor (β3AR) in brown adipose tissue. As a result, mRNA expression of UCP-1 significantly increased in the KPE 1 % treatment group, indicating that KPE activated brown adipose tissue. We then evaluated the direct effects of KPE on brown adipocytes using primary brown preadipocytes isolated from interscapular brown adipocytes in ICR mice. Triacylglycerol (TG) accumulation in primary brown preadipocytes was increased by KPE in a dose-dependent manner. Each mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ), UCP-1, and β3AR exhibited an upward trend compared with the control group. Moreover, some polymethoxyflavonoids (PMFs), the main compound in KP, also increased TG accumulation. This study therefore showed that KPE enhanced the thermogenesis effect of brown adipocytes as well as promoted the differentiation of brown adipocyte cells.

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    Authors and Affiliations

    1. Graduate School of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo, 142-8501, Japan

      Hiroko Kobayashi & Kiyoshi Sugiyama

    2. Faculty of Pharmacy, Musashino University, 1-1-20 Shinmachi, Nishitokyo-shi, Tokyo, 202-8585, Japan

      Emi Horiguchi-Babamoto, Mio Suzuki, Tsutomu Shimada & Masaki Aburada

    3. Department of Molecular Pharmacology and Neurobiology, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan

      Hiroko Makihara

    4. Research and Development Division, Toyo Shinyaku Co., Ltd., 7-28 Yayoigaoka, Tosu-shi, Saga, 841-0005, Japan

      Hiroshi Tomozawa & Masahito Tsubata

    5. Department of Hospital Pharmacy, University Hospital, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan

      Tsutomu Shimada

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    1. Hiroko Kobayashi
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    Correspondence to Tsutomu Shimada.

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    Hiroko Kobayashi and Emi Horiguchi-Babamoto contributed equally to this work.

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    Kobayashi, H., Horiguchi-Babamoto, E., Suzuki, M. et al. Effects of ethyl acetate extract of Kaempferia parviflora on brown adipose tissue. J Nat Med 70, 54–61 (2016). https://doi.org/10.1007/s11418-015-0936-2

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    • DOI: https://doi.org/10.1007/s11418-015-0936-2

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