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Thoracic Vagal Efferent Nerve Stimulation Evokes Substance P-Induced Early Airway Bronchonstriction and Late Proinflammatory and Oxidative Injury in the Rat Respiratory Tract

  • Published:
Journal of Biomedical Science

Summary

Electrical stimulation of efferent thoracic vagus nerve (TVN) evoked neurogenic inflammation in respiratory tract of atropine-treated rats by an undefined mechanism. We explored whether efferent TVN stimulation via substance P facilitates neurogenic inflammation via action of nuclear factor-κB (NF-κB) activation and reactive oxygen species (ROS) production. Our results showed that increased frequency of TVN stimulation concomitantly increased substance P-enhanced hypotension, and bronchoconstriction (increases in smooth muscle electromyographic activity and total pulmonary resistance). The enhanced SP release evoked the appearance of endothelial gap in silver-stained leaky venules, India-ink labeled extravasation, and accumulations of inflammatory cells in the respiratory tract, contributing to trachea plasma extravasation as well as increases in blood O −2 and H2O2 ROS amount. L-732138 (NK1 receptor antagonist), SR-48968 (NK2 receptor antagonist), dimethylthiourea (H2O2 scavenger) or catechins (O −2 and H2O2 scavenger) pretreatment reduced efferent TVN stimulation-enhanced hypotension, bronchoconstriction, and plasma extravasation. Increased frequency of TVN stimulation significantly upregulated the expression of nuclear factor-κB (NF-κB) in nuclear protein and intercellular adhesion molecule-1 (ICAM-1) in total protein of the lower respiratory tract tissue. The upregulation of NF-κB and ICAM-1 was attenuated by NK receptor antagonist and antioxidants. In conclusion, TVN efferent stimulation increases substance P release to trigger NF-κB mediated ICAM-1 expression and O −2 and H2O2 ROS production in the respiratory tract.

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Authors and Affiliations

  1. Department of Biological Science, National Sun Yat-Sen University, Kaohsiung, ROC

    Ping-Chia Li & Chen-Fu Shaw

  2. Department of Electronics Engineering, Yung-Ta Institution of Technology and Commerce, Ping-Don, ROC

    Sheng-Chung Li

  3. Department of Family Medicine, National Cheng-Kung University Hospital, Tainan, ROC

    Yuan-Ju Lin

  4. Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, ROC

    Jin-Tung Liang

  5. Department of Medical Research, National Taiwan University Hospital and National Taiwan University College of Medicine, 7 Chung-Shan S. Road, Taipei, Taiwan, ROC

    Chiang-Ting Chien

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  1. Ping-Chia Li
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Correspondence to Chiang-Ting Chien.

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Li, PC., Li, SC., Lin, YJ. et al. Thoracic Vagal Efferent Nerve Stimulation Evokes Substance P-Induced Early Airway Bronchonstriction and Late Proinflammatory and Oxidative Injury in the Rat Respiratory Tract. J Biomed Sci 12, 671–681 (2005). https://doi.org/10.1007/s11373-005-7892-x

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  • DOI: https://doi.org/10.1007/s11373-005-7892-x

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