Abstract
A Trichoderma orientale strain LSBA1 was isolated from the Mediterranean marine sponge Cymbaxinella damicornis. The crude extract of T. orientale mycelium showed inhibitory activity against growth of Gram-positive and Gram-negative bacteria as well as clinical isolates of Candida albicans. Purification of the anti-Candida component was performed using a combination of open silica gel-60 column and reverse phase high performance liquid chromatography. The active compound called hyporientalin A has been identified as a peptaibol analogue of longibrachin-A-II using mass spectrometry. It exhibited fungicidal activity against clinical isolates of C. albicans with minimal inhibitory concentrations (MICs) ranging from 2.49 to 19.66 µM, comparable to that of the antifungal agent amphotericin B. Our data support the use of hyporientalin A as a promising new and efficient antifungal drug in the treatment of candidiasis while controlling toxicity.
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Acknowledgements
We thank Mr. Rafed Daldoul from Diving Center Cap Afrique, Mahdia, Tunisia for helpful Scuba diving; we thank Dr. Perez from “Institut Méditerranéen de la Biodiversité et d’Ecologie Marine et Continentale, Marseille, France” for helpful comments and identification of Cymaxinella damicornis. We also wish to thank Dr. Yann Guitton for his technical contribution and Prof. E. Aouani for valuable discussion and critical reading of the manuscript. This study was supported by the “Ministère de l’Enseignement Supérieur and the project ECIMAR (French ANR in Biodiversity, 2006)”.
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Touati, I., Ruiz, N., Thomas, O. et al. Hyporientalin A, an anti-Candida peptaibol from a marine Trichoderma orientale. World J Microbiol Biotechnol 34, 98 (2018). https://doi.org/10.1007/s11274-018-2482-z
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DOI: https://doi.org/10.1007/s11274-018-2482-z