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Molecular characterization and phylogeny of two begomoviruses infecting Malvastrum americanum in Jamaica: evidence of the contribution of inter-species recombination to the evolution of malvaceous weed-associated begomoviruses from the Northern Caribbean

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Abstract

Two distinct full-length begomovirus DNA-A components and a DNA-B component were PCR amplified, cloned and sequenced from Jamaican Malvastrum americanum plants exhibiting yellow mosaic symptoms. Whereas one of the DNA-A components is from a potentially new species that we have tentatively named Malvastrum yellow mosaic Helshire virus (MaYMHV), the other DNA-A and the DNA-B form a cognate pair and represent a new virus species tentatively named Malvastrum yellow mosaic Jamaica virus (MaYMJV). The MaYMJV genome components together infected M. americanum and produced yellow mosaic symptoms similar to those seen in naturally infected plants. Both the MaYMJV and MaYMHV DNA-A components are typical of those of bipartite begomoviruses from the Western Hemisphere. The DNA-As of MaYMJV and MaYMHV are most closely related to each other (sharing 84% sequence identity) and cluster phylogenetically with begomoviruses found infecting malvaceous weeds in Cuba and Florida. The DNA-B component of MaYMJV is most similar to that of Sida golden mosaic virus-[USA:Florida] (SiGMV-[US:Flo]) and Sida golden mosaic Costa Rica virus-[Costa Rica] (SiGMCRV-[CR]). As with many other geminivirus species, the genomes of MaYMJV and MaYMHV bear traces of inter-species recombination.

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Acknowledgements

Funding for this work was provided by The Principal’s New Initiative Fund and The School of Graduate Studies and Research at the University of the West Indies (Mona campus). The authors are also grateful for the photographic assistance of Mr. Darshan Young and for his help along with Miss Cheryl Stewart in gathering DNA sequence data for the recombination analysis.

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Correspondence to André P. Graham.

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These files are unfortunately not in the Publisher's archive anymore:

  • DNA-B.rdp RDP project files that can be opened in the program RDP3 (which may be downloaded from http://darwin.uvigo.es/rdp/rdp.html) and used to interactively explore the patterns of recombination evident among Western Hemisphere begomovirus DNA-B sequences (RDP 965 kb)

  • DNA-A.rdp RDP project files that can be opened in the program RDP3 and used to interactively explore the patterns of recombination events among Western Hemisphere begomovirus DNA-A sequences (RDP 432 kb)

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Graham, A.P., Martin, D.P. & Roye, M.E. Molecular characterization and phylogeny of two begomoviruses infecting Malvastrum americanum in Jamaica: evidence of the contribution of inter-species recombination to the evolution of malvaceous weed-associated begomoviruses from the Northern Caribbean. Virus Genes 40, 256–266 (2010). https://doi.org/10.1007/s11262-009-0430-6

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  • DOI: https://doi.org/10.1007/s11262-009-0430-6

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