Abstract
Echinococcus granulosus sensu lato causes Cystic echinococcosis. This study investigated the bacterial and fungal species in the liver and lung hydatid cysts obtained from sheep, goats, cattle, and camels slaughtered in Yazd abattoir, Central Iran. In this study, 84 hydatid cysts were obtained from 20 sheep, 13 goats, 25 cattle, and 26 camels. The fertility and viability rates were assessed using light microscopy and eosin staining, respectively. The aspirated hydatid cysts were cultured to detect the presence of any bacteria and fungi. Bacterial isolates were identified by biochemical tests. DNA was also extracted from germinal layers, and then genotyping was carried out targeting the cox 1 gene. The statistical analysis was performed by SPSS version 16.0. This study showed that 22.62% (19/84) of hydatid cysts had bacterial occurrence, and none of the samples had fungal species. Among the fertile cysts, 52.6% had bacterial occurrence, of which 40% were viable. Most bacteria detected in hydatid cysts included Staphylococcus saprophyticus, Escherichia coli, and S. epidermidis. Hydatid cysts with bacterial occurrence were identified as G1-G3, G5, and G6/G7. The bacterial species occurrence in hydatid cysts had no significant relationship with fertility and viability (P > 0.05), without any significant relation with viability (P > 0.05), animal species (P > 0.05), involved organ in animals (P > 0.05), and hydatid cyst genotypes (P > 0.05). It should also be mentioned that this is the first study to assess the relationship between hydatid cyst genotyping and the occurrence of fungal and bacterial species.
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Data availability
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This research was supported by Shahid Sadoughi University of Medical Sciences, Yazd, Iran (grant number: 6361). We would like to sincerely thank Mr. Salman Ahmadian and Mr. Ahmad Baghbani for their support in sample collection.
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We would like to thank Shahid Sadoughi University of Medical Sciences, Yazd, Iran for financial supports with the grant number of 6361.
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All authors contributed to the study conception and design. First draft writing and data collection: [Sajad Zandi]; conceptualization and edition: [Mara Mariconti]; Visualization and conceptualization: [Hengameh Zandi]; Data curation, validation and supervised the methodology: [AbbasAli Jafari] and [Bahador Hajimohammadi]; Project administration, supervision and edition: [Gilda Eslami]; Formal and statistical analysis: [Mahmood Vakili]; Material preparation and methodology: [Maryam Sheykhzadegan] and [Vahideh Askari]; the methodology: [Saeedeh Sadat Hosseini]. all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Zandi, S., Mariconti, M., Zandi, H. et al. Bacterial and Fungal Occurrence in Hydatid Cysts from Livestock in Central Iran. Vet Res Commun 47, 421–430 (2023). https://doi.org/10.1007/s11259-022-09959-8
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DOI: https://doi.org/10.1007/s11259-022-09959-8