Abstract
The high lipid content in porcine oocytes impairs in vitro embryo production (IVP). Here, we evaluated the influence of two different lipid modulators during in vitro maturation (IVM) on the embryo development and the lipid content of oocytes and embryos. In Experiment I, oocytes were exposed to 50 μM docosahexaenoic acid (DHA) with (+) or without (−) the presence of porcine follicular fluid (pFF). In Experiment II, phenazine ethosulfate (PES) was added during IVM at two concentrations (0.5 and 0.05 μM). The pFF- with 50 μM DHA treatment impaired nuclear maturation, cleavage and blastocyst rates (p < 0.05). Oocytes in pFF- media accumulated less lipids (p < 0.05). The addition of 0.5 μ M PES reduced all development rates (p < 0.05) and resulted in higher lipid content for oocytes and embryos. Only 0.05 μM PES oocytes matured similarly to the control (p > 0.05), although embryo development and embryo lipid content was similar to 0.5 μM PES oocytes (p > 0.05). Thus, 50 μM DHA supplementation in the IVM medium without pFF impaired oocyte maturation and embryo development rates without interfering in oocyte lipid content even in the presence of pFF. Maturation with PES neither favored porcine embryo development nor reduced their lipid content.
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Data availability
The datasets in this study are available from the corresponding author on reasonable request. All data and materials are available for publication.
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Acknowledgments
The authors thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo a Pesquisa do Rio Grande do Sul (FAPERGS) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Finance Code 001), which jointly financed this study, to Laboratórios Microvet for the financial support given to the first author and to Frigorífico Castro (Pelotas-RS) for allowing the collection of the ovaries used in this study.
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José Victor Cardoso Braga – Investigation, data curation, visualization and writing – original draft; Andrez Pastorello Bohn – Investigation; Luana Pescke Soares – Investigation; Eliza Rossi Komninou - Data curation, investigation, resources, writing – original draft; Thomaz Lucia Júnior – Formal analysis, funding acquisition, resources, writing – review & editing; Arnaldo Diniz Vieira – Conceptualization, methodology, resources, supervision, validation, writing – review & editing; Rafael Gianella Mondadori – Conceptualization, funding acquisition, methodology, project administration, resources, supervision, validation, writing – review & editing.
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The ovaries were collected in a slaughterhouse inspected by the Federal Inspection Service from the Ministry of Agriculture, Livestock and Supply, where all humanitarian slaughter regulations are followed and inspected. As the experiments does not involve animal models, the institution does not require approval by the Ethics and Animal Experimentation Committee.
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Braga, J.V.C., Bohn, A.P., Soares, L.P. et al. Docosahexaenoic acid and phenazine ethosulfate are not efficient lipid modulators for porcine in vitro maturation systems. Vet Res Commun 46, 731–738 (2022). https://doi.org/10.1007/s11259-022-09889-5
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DOI: https://doi.org/10.1007/s11259-022-09889-5